CircularRNA CDR1as promotes osteogenic differentiation and angiogenesis related genes expression in mouse bone marrow mesenchymal stem cells
10.12016/j.issn.2096-1456.2022.06.002
- Author:
YANG Weizhe
1
;
HAN Xiangzhen
1
;
ZHENG Meijie
1
;
ZHOU Qiqi
1
;
HE Huiyu
2
Author Information
1. Department of Prosthodontics, the First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University
2. 1.Department of Prosthodontics, the First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University 2.Xinjiang Uygur Autonomous Region Institute of Stomatology
- Publication Type:Journal Article
- Keywords:
bone;
CircularRNA CDR1as;
gene silencing;
mouse bone marrow mesenchymal stem cells;
runt-related transcription factor 2;
collagen-I;
vascular endothelial growth factor;
osteogenic differentiation;
bone tissue engineering
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2022;30(6):390-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of over expression and low expression of antisense transcripts of circular RNA cerebellar degeneration associated protein 1 (CDR1as) in Balb/C mouse bone marrow mesenchymal stem cells (BMSCs) on factors related to osteogenesis and angiogenesis.
Methods:BMSCs were cultured and identified in vitro. The lentiviral (LV) vector containing the overexpressed and silenced circRNA CDR1as genes and the control lentivirus were respectively transfected into mouse BMSCs, and stable cell lines were screened. The cells were divided into the circRNACDR1as over expression group and the over expression control group, and the CircRNACDR1as low expression group and the low expression control group. The components were stained with Alizarin Red S and alkaline phosphatase after 14 and 21 days of osteoinduction; qRT-PCR was used to detect the target genes circRNA CDR1as, osteogenic differentiation markers alkaline phosphatase (ALP), runt- related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN), osterix(Osx), collagen I (COL-1), and the mRNA expression levels of vascular endothelial grown factor (VEGF) and angiogenin-1 (Ang-1).
Results: The results of alizarin red staining and alkaline phosphatase staining showed that the extracellular matrix calcium precipitation and ALP staining area of the over expression experimental group was greater than its control group, and those of the low expression experimental group was less than its control group. As the number of days of osteogenic induction increased, the calcium precipitation and ALP staining in each group also increased. RT-PCR results showed that the mRNA expression levels of circRNA CDR1as, ALP, RUNX2, OCN, OPN, OSX, COL-1, VEGF and Ang-1 in the over expression experimental group BMSCs were significantly increased (P<0.001). In the low expression experimental group, the mRNA expression levels of circRNA CDR1as, ALP, RUNX2, OCN, OPN, OSX, COL-1, VEGF and Ang-1 in BMSCs were significantly reduced (P<0.001).
Conclusion: Over expression of the circRNA CDR1as gene promotes the osteogenic differentiation and angiogenesis of BMSCs. Low expression of the circRNA CDR1as gene inhibits the osteogenic differentiation and angiogenesis of BMSCs.
- Full text:环状RNA CDR1as促进小鼠骨髓间充质干细胞成骨分化和成血管相关基因表达.pdf
