Establishment of a mouse model of vascular endothelial-mesenchymal transdifferentiation genetic tracing and its role in liver fibrosis studies
10.3969/j.issn.1001-5256.2022.04.018
- VernacularTitle:血管内皮间质样转分化遗传示踪小鼠模型的构建及其在肝纤维化研究中的应用
- Author:
Hao XU
1
;
Bai RUAN
1
,
2
;
Zhiwen LI
1
;
Zhiqiang FANG
1
;
Lin WANG
1
;
Kefeng DOU
1
Author Information
1. Department of Hepatobiliary, Pancreatic and Splenic Surgery, Xijing Hospital, Air Force Medical University, Xi'an 710032, China
2. Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Air Force Medical University, Xi'an 710032, China
- Publication Type:Original Articles_Liver Fibrosis and Liver Cirrhosis
- Keywords:
Liver Cirrhosis;
Epithelial-Mesenchymal Transition;
Endothelial Cells;
Mice
- From:
Journal of Clinical Hepatology
2022;38(4):832-836
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish Cdh5-Cre ERT /Acta2-tdTomato-STOP floxed -eGFP knockin genetic tracing mice, and to investigate its application in studies on vascular endothelial cell transition in liver fibrosis. Methods Cdh5-Cre ERT mice were mated with Acta2-KI mice, and the Cdh5-Cre ERT /Acta2-KI genetic tracing mice were obtained and identified by PCR genotyping. Primary liver sinusoid endothelial cells (LSECs) were isolated and cultured, and a model of CCl 4 -induced liver fibrosis was established. LSECs and liver tissue were collected for immunofluorescent staining to observe the expression of the fluorescent proteins tdTomato and eGFP. Results After being induced by tamoxifen, LSECs and liver tissue of Cdh5-Cre ERT /Acta2-KI genetic tracing mice expressed eGFP under the conditions for epithelial-mesenchymal transdifferentiation established in vivo and in vitro, while the control group without induction expressed tdTomato alone. Conclusion The successfully established Cdh5-Cre ERT /Acta2-KI genetic tracing mice can realize the effective labeling of epithelial-mesenchymal transdifferentiation, which provides a genetic tracing basis for the diverse sources of mesenchymal myofibroblasts in liver fibrosis.
