Full spectrum analysis of chemical constituents of Sargassum fusiforme and its in vitro anti-neuroinflammatory activity
- VernacularTitle:海藻羊栖菜化学成分全谱分析及其体外抗神经炎症活性研究
- Author:
Hong PENG
1
,
2
,
3
;
Pinzhe HUANG
1
,
3
;
Yonggui SONG
2
,
3
;
Huanhua XU
2
,
3
,
4
;
Mingyue ZHOU
2
,
3
;
Genhua ZHU
2
,
3
;
Ming YANG
4
;
Zhifu AI
2
,
3
;
Dan SU
1
,
2
Author Information
1. College of Pharmacy，Jiangxi University of Chinese Medicine，Nanchang 330004，China
2. Key Laboratory of Depression Animal Model Based on TCM Syndrome of Jiangxi Administration of Traditional Chinese Medicine，Jiangxi University of Chinese Medicine，Nanchang 330004，China
3. Jiangxi Provincial Key Laboratory of TCM for Prevention and Treatment of Brain Diseases with Cognitive Impairment，Jiangxi University of Chinese Medicine，Nanchang 330004，China
4. Key Laboratory of Modern Preparation of Chinese Medicine of Ministry of Education，Jiangxi University of Chinese Medicine，Nanchang 330004，China
- Publication Type:Journal Article
- Keywords:
Sargassum fusiforme;
full spectrum analysis
- From:
China Pharmacy
2022;33(7):800-807
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the composition of chemical constituents of Sargassum fusiforme and its in vitro anti- neuroinflammatory activity ，and to provide reference for its development and utilization and the study of pharmacodynamic substances. METHODS UHPLC-QTOF-MS/MS analysis method and GC-MS/MS method were used to analyze the chemical constituents of S. fusiforme . The lipopolysaccharide （1 μg/mL）was adopted to establish the inflammatory model of neuromicroglia BV2. Using paroxetine （5 μg/mL）as positive control ，CCK-8 assay was used to detect the effects of the extracts of S. fusiforme （20，40，60，80，100 μg/mL）on the activity and morphology of neuromicroglia BV 2. The effects of the extracts of S. fusiforme （40，60，80 μg/mL）on the contents of tumor necrosis factor α（TNF-α）and interleukin- 6（IL-6）in cell supernatant were detected by ELISA. RESULTS A total of 103 non-volatile constituents were identified by UHPLC-QTOF-MS/MS ，and 60 volatile constituents were obtained by GC-MS/MS. The extracts of S. fusiforme （40，60，80 μ g/mL） could significantly reduce the abnormally increased activation of neuromicroglia BV 2 and the contents of TNF-α and IL-6 due to lipopolysaccharide （P＜0.05 or P＜0.01）. CONCLUSIONS The study establish the full spectrum of chemical constituents of S. fusiforme ，and it is confirmed that fusiforme has certain in vitro anti-neuroinflammatory activity.
