Polymorphism analysis on the 5′ flanking region of CD25 gene of Han males in naturally high radiation background area
10.11763/j.issn.2095-2619.2021.03.016
- Author:
Ying TANG
1
;
Wei-jun TAN
1
;
Ya-li JIN
;
Shi-biao SU
;
Cui-ju WEN
Author Information
1. School of Public Health, Guangdong Pharmaceutical University Guangzhou, Guangdong 510310, China
- Publication Type:Journal Article
- Keywords:
High radiation background area;
Gene polymorphism;
Gene frequency;
Alleles;
Genotype;
Genetic variability
- From:
China Occupational Medicine
2021;48(03):324-328
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To explore the distribution characteristics of single nucleotide polymorphism(SNP) rs7072793 and rs3118470 in the 5′ flanking region of(cluster of differentiation 25, CD25) gene in Han males in the naturally high radiation background area(HBRA). METHODS: A random sampling method was used to select 48 and 51 healthy Han males from Tangkou town(HBRA group) in Yangjiang City and Hengpo town(control group) in Enping City, respectively, as the study subjects. The molecular mass array method was used to classify the genotype of the SNP sites rs7072793 and rs3118470 of CD25 gene in these subjects. The distribution difference of the alleles and genotypes was analyzed in individuals of these two groups. The allele frequency of HBRA population was compared with the distribution data of different populations in the Human Genome Project.RESULTS: The distribution of allele frequencies of rs7072793 and rs3118470 in both groups were consistent with the H-W equilibrium law of genetics(all P>0.05). In the HBRA group, variant allele C(58.3%) and genotype TC(50.0%) were dominant at rs7072793, wild-type allele T(55.2%) and genotype TC(56.2%) were dominant at rs3118470. There was no significant difference in the allele and genotype distributions between these two groups(all P>0.05). There was a difference of rs7072793 in the HBRA group compared to that of African and European populations, and rs3118470 in the HBRA group compared with the allele distribution frequencies in Africa, Europe and America populations(all P<0.05). CONCLUSION: In the male population of Han nationality in Yangjiang HBRA area, the alleles of rs7072793 and rs3118470 in the 5′ flanking region of CD25 gene were mainly C and T, respectively, and the genotypes were mainly TC. These two loci may have high genetic variability.
