UHPLC-Q-TOF/MS analysis of chemical constituents in compound Jinqiancao granules
10.12206/j.issn.1006-0111.202105117
- VernacularTitle:复方金钱草颗粒化学成分的UHPLC-Q-TOF/MS分析
- Author:
Zhe ZHANG
1
;
Zhuoran SUN
2
;
Pengchao PAN
3
;
Xiaofei CHEN
2
;
Yifeng CHAI
2
Author Information
1. Department of Pharmacy, No. 971 Naval Hospital of the PLA, Qingdao 266071, China.
2. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
3. The First Naval Hospital of Southern Theater Command of the PLA, Zhanjiang 524005, China.
- Keywords:
compound Jinqiancao granules;
UHPLC-Q-TOF/MS;
chemical constituents
- From:
Journal of Pharmaceutical Practice
2022;40(2):146-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To qualitatively analyze the main chemical components in compound Jinqiancao granules by ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Methods XBridge BEH C18 column (2.1 mm×100 mm, 2.5 µm) was used for chromatographic separation. The mobile phase was composed of 0.1% formic acid water and 0.1% formic acid-acetonitrile, gradient elution, and the flow rate was 0.4 ml/min. Mass spectrometry was characterized by Quadrupole time-of-flight mass spectrometry (Q-TOF/MS) and positive ion mode scanning. Results Under the optimized LC/MS condition, 47 components in compound Jinqiancao granules were identified. The isomers were distinguished by software calculation. The source of medicinal materials was assigned. Conclusion A rapid and efficient analytical method was established for the identification of chemical components in compound Jinqiancao granules by UHPLC-Q-TOF/MS.
