Effect of Bixie deacidification fang on hyperuricemia mouse model and its effect on the expression of renal urate transporter
10.12206/j.issn.1006-0111.202101017
- VernacularTitle:萆薢降酸方对高尿酸血症小鼠模型的药效作用及其对肾脏尿酸盐转运体表达的影响
- Author:
Qiong WANG
1
;
Bing ZHOU
1
;
Quanwei YANG
2
Author Information
1. Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430000, China.
2. Wuhan No.1 Hospital, Wuhan 430000, China.
- Keywords:
Bixie deacidification fang;
hyperuricemia;
renal urate transporters;
uric acid;
creatinine
- From:
Journal of Pharmaceutical Practice
2022;40(2):143-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-hyperuricemia effects of Bixie deacidification fang on hyperuricemia mice and its mechanism of renal protein transport. Methods The effects of Bixie deacidification fang were investigated on hyperuricemia mice induced by potassium oxonate. Bixie deacidification fang was administered to hyperuricemia mice daily at doses of 220, 440 and 880 mg/kg for 10 days, and allopurinol (5mg/kg) was given as positive control. Serum and urine levels of uric acid and creatinine were determined by colorimetric method. Simultaneously, protein levels of urate transporter 1 (URAT1) and organic anion transporter 1 (OAT1) in the kidney were analyzed by Western blot. Results Compared with the model group, high-dose of Bixie deacidification fang inhibited xanthine oxidase (XOD) activities in serum (18.12±1.33 u/L) and that in liver (70.15±5.20 u/g protein) (P<0.05), decrease levels of serum uric acid (2.04 ± 0.64mg/L) (P<0.05) and serum creatinine (0.35±0.18µmol/L) and blood urea nitrogen (BUN)(8.83±0.71mmol/L) (P<0.05), ncreased levels of urine uric acid (38.34±8.23mg/L), urine creatinine (34.38±1.98mmol/L), down-regulated of URAT1 and up-regulated of OAT1 protein expressions (P<0.05) in the renal tissue of hyperuricemia mice. Conclusion Bixie deacidification fang recipe may promote the excretion of uric acid in the kidney by up-regulating the expression of OAT1 protein to promote the excretion of uric acid, and down-regulating the expression of URAT1 protein to inhibit the reabsorption of uric acid.
