Potential molecular mechanism of motherwort in the treatment of nerve injury based on network pharmacology
10.12206/j.issn.1006-0111.202105106
- VernacularTitle:基于网络药理学的益母草治疗神经损伤的潜在分子机制研究
- Author:
Qian ZHANG
1
,
2
;
Yuchen GUO
1
,
2
;
Shanshan DENG
3
;
Yuefan ZHANG
3
;
Tiejun LI
1
,
2
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2. Punan Hospital, Pudong New Area, Shanghai 200125, China.
3. School of Medicine, Shanghai University, Shanghai 200444, China.
- Keywords:
motherwort;
network pharmacology;
nerve injury
- From:
Journal of Pharmaceutical Practice
2022;40(2):113-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of motherwort in the treatment of nerve injury. Methods The active components of motherwort were obtained by searching TCMSP and BATMAN-TCM databases. The action targets of candidate compounds were collected and predicted from TCMSP and SwissTargetPrediction (STP) databases. The target genes corresponding to the active components of motherwort were obtained by using the standardized database of disease targets (Uniprot). The potential targets of motherwort in the treatment of nerve injury were obtained by mapping the disease genes of nerve injury with the three databases of Genecards, DisGenet and OMIM. The network topology analysis software Cytoscape 3.6.0 was used to construct the action target network of motherwort active components. The protein interaction platform database (STRING) was used to construct the interaction relationship between action targets. The target protein interaction (PPI) network was constructed by introducing Cytoscape 3.6.0 software. Through STRING database, GO enrichment analysis and KEGG pathway enrichment analysis were carried out to analyze the target points of motherwort in the treatment of nerve injury. Results 19 active components were screened from motherwort, involving 654 action targets, including 426 action targets related to nerve injury and 6 key targets. These target genes were mainly involved in biological regulation, oxidative stress response and cell communication and other biological processes. Molecular functions were mainly related to protein binding, ion binding and catalytic reduction. They were enriched outside the cell membrane. Its mechanism was related to signal pathways such as MAPK, Toll-like receptor, PI3K-Akt, TNF, IL-17, and apoptosis. Conclusion The active components of motherwort may play a protective role on nerve injury through anti-inflammation, anti-apoptosis and promoting cell growth.
