Simultaneous determination of 7 components in Dipsacus asper by HPLC
- VernacularTitle:HPLC法同时测定续断药材中7个成分的含量
- Author:
Changgui YANG
1
;
Anhui GONG
2
;
Chenggang ZHANG
1
;
Chenghong XIAO
1
;
Di FAN
3
;
Tao ZHOU
1
Author Information
1. Institute of Traditional Chinese Medicine and Ethnic Medicine Resources，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
2. Dept. of Pharmacy，Guizhou Health Vocational College，Guizhou Tongren 554300，China
3. Guizho u Crop Technology Promotion Station，Guiyang 550001，China
- Publication Type:Journal Article
- Keywords:
Dipsacus asper;
content determination;
high performance liquid chromatography;
triterpene saponins;
iridoids
- From:
China Pharmacy
2022;33(6):680-684
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establis h the method for the simultaneous determination of six iridoids （loganic acid ，loganin， sweroside，dipsanoside B ，dipsanoside A ，sylvestroside Ⅰ）and one triterpene saponin （asperosaponin Ⅵ）in Dipsacus asper . METHODS High performance liquid chromatography （HPLC） method was adopted. The determination was performed on Symmetry® C18 column with mobile phase consisted of acetonitrile- 0.1％ phosphoric acid solution （gradient elution ）at the flow rate of 1.0 mL/min. The detection wavelengths were set at 212 nm（asperosaponin Ⅵ）and 237 nm（dipsanoside B ，dipsanoside A ， sweroside，loganic acid ，sylvestroside Ⅰ，loganin）. The column temperature was set at 30 ℃，and sample size was 20 μL. RESULTS The linear range of loganic acid ， loganin， sweroside， sylvestroside Ⅰ ， dipsanoside B ， dipsanoside A and asperosaponin Ⅵ were 399.24-931.56，50.30-150.90，48.24-168.84，27.00-70.20，12.93-38.80，40.64-121.92，42.08-147.28 µg/mL （all r＞0.999 0）. RSDs of precision ，reproducibility and stability tests （24 h）were all less than 2％. Average recoveries were 104.43％（RSD＝0.63％，n＝6），101.74％（RSD＝1.11％，n＝6），100.76％（RSD＝1.06％，n＝6），98.00％（RSD＝1.58％，n＝6）， 99.03％（RSD＝2.31％，n＝6），102.93％（RSD＝2.26％，n＝6），102.31％（RSD＝1.00％，n＝6），respectively，The contents were 142.5-280.6，5.5-49.0，28.0-112.9，7.2-35.8，4.4-16.9，17.2-79.3，0.8-54.5 mg/g，respectively. CONCLUSIONS Established method is accurate and reliable ，and can be used for the content determination of 7 components in D. asper .
