Piceatannol alleviates host inflammation in chronic kidney disease model mice through regulating gut microbiota
10.16438/j.0513-4870.2021-0990
- VernacularTitle:白皮杉醇调控肠道菌群减轻慢性肾病模型小鼠并发系统性炎症
- Author:
Cheng-xi LI
;
Ying-yi WANG
;
Yu-meng WANG
;
Jia-ting YIN
;
Shu-hui YANG
;
Yun LIU
;
Jin-ao DUAN
;
Jian-ming GUO
- Publication Type:Research Article
- Keywords:
chronic kidney disease;
host inflammation;
piceatannol;
gut microbiota;
uremic toxin
- From:
Acta Pharmaceutica Sinica
2022;57(2):364-374
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this research is to study the effect of small molecule compound piceatannol (PIC) on host inflammation in adenine induced chronic kidney disease (CKD) mice, and then to explore its mechanism based on the regulation of gut microbiota. All procedures were approved by the Institutional Animal Care and Use Committee of the Nanjing University of Chinese Medicine. The level of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was detected by enzyme linked immunosorbent assay (ELISA); UPLC-TQ/MS technology was used to monitor the level of proinflammatory uremic toxin indoxyl sulfate (IS) and p-cresol sulfate (PCS); the expression of occludin was tested by Western blot; in vitro anaerobic culture of gut bacteria was used to produce indole; the abundance of gut microbiota was evaluated by 16S rDNA sequencing. The results showed that PIC had no effect on inflammatory infiltration in kidney tissue of CKD mice, but could decrease IL-6 level in blood and IL-6/TNF-α level in colon tissue. PIC did not improve intestinal occludin protein expression in CKD mice; while it could significantly reduce the levels of IS and PCS in blood and liver of CKD mice. Further mechanism studies showed that PIC could inhibit the synthesis of IS precursor indole in gut bacteria. Moreover, PIC could decrease the abundance of gut bacteria which producing uremic toxin, such as reducing the abundance of indole and p-cresol producing gut bacteria. In conclusion, PIC could regulate gut microbiota and inhibit the synthesis of uremic toxin precursor, thereafter reducing the accumulation of IS and PCS in vivo, ultimately relieving the inflammation of CKD mice.
