Q-marker prediction analysis of Gentiana rigescens Franch. based on HPLC and network pharmacology
10.16438/j.0513-4870.2021-1147
- VernacularTitle:基于液相色谱及网络药理学的滇龙胆质量标志物 (Q-marker) 预测分析
- Author:
Chun-lu LIU
1
,
2
;
Tao SHEN
3
,
4
;
Ji ZHANG
3
;
Fu-rong XU
1
;
Yuan-zhong WANG
3
Author Information
1. College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, China
2. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China
3. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China
4. College of Chemistry, Biology and Environment, Yuxi Normal University, Yuxi 653100, China
- Publication Type:Research Article
- Keywords:
italic>Gentiana rigescens;
quality marker (Q-marker);
network pharmacology;
chemical fingerprint;
chemical constituent
- From:
Acta Pharmaceutica Sinica
2022;57(3):766-774
- CountryChina
- Language:Chinese
-
Abstract:
Aa a characteristic medicinal plant in China, Gentiana rigescens Franch. has the function of protecting the liver and invigorating the spleen. At present, there are a few studies on the content determination method of characteristic components of G. rigescens, so it is necessary to establish a scientific and effective quality control method; In this study, The high performance liquid chromatography (HPLC) fingerprint of G. rigescens was established, based on literature reviewed and characteristic spectrum identified, the source range of G. rigescens quality marker (Q-marker) was screened. The effectiveness of the ingredients and the corresponding targets and pathways was analyzed through network pharmacology, and drew the diagram of ''component-target-pathway''. Qualitative and quantitative analysis of G. rigescens was performed by HPLC, and screen the main marker components leading to the differences between groups which were determined the Q-marker of G. rigescens; The literature and HPLC had determined that five iridoids were the main source of G. rigescens Q-marker. The network pharmacology (effectiveness) and qualitative and quantitative (detectability) analysis of G. rigescens from different producing areas confirmed that gentiopicroside, swertiamarin, and sweroside can be used as the main landmark components, and there were significant differences in their contents among different producing areas; The analysis of G. rigescens from different producing areas was carried out by network pharmacology and chemical fingerprints, it is confirmed can be used as potential Q-marker to provide sufficient theoretical basis for the quality control of G. rigescens in the later period.