Controlled release and cytobiology of recombinant human parathyroid hormone 1-34 mediated by hyaluronic acid thermo-sensitive hydrogels
10.16438/j.0513-4870.2021-0999
- VernacularTitle:透明质酸温敏水凝胶介导的重组人甲状旁腺激素 (1-34) 可控释放与细胞生物学研究
- Author:
Shao-yan XUAN
1
;
Yue ZHU
2
,
3
;
Lin ZHANG
1
;
Zhi-hua TANG
1
;
Feng XU
2
;
Teng CHEN
2
;
Zu-hua WANG
2
,
3
Author Information
1. Shaoxing People's Hospital, Shaoxing 312000, China
2. Nano-drug Technology Research Center, College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
3. Guizhou Engineering Technology Research Center for Processing and Preparation of Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China
- Publication Type:Research Article
- Keywords:
hyaluronic acid;
thermo-sensitive hydrogel;
rug sustained and controlled release;
osteoclast;
recombinant human parathyroid hormone (1-34)
- From:
Acta Pharmaceutica Sinica
2022;57(3):809-817
- CountryChina
- Language:Chinese
-
Abstract:
The bone formation promoter recombinant human parathyroid hormone 1-34 [PTH (1-34)] has a short half-life and low bioavailability. In this study, we prepared a biodegradable and temperature-sensitive hyaluronic acid-poly-N-isopropyl acrylamide (AHA-g-PNIPAAm), and further investigated its effects of PTH (1-34) release and cell behavior as drug carrier. The structure of AHA-g-PNIPAAM was confirmed by hydrogen nuclear magnetic resonance spectroscopy and infrared spectroscopy. Next, PTH (1-34) loaded thermo-sensitive hydrogels were prepared by physical swelling method and their stability was investigated. The morphology of hydrogel was observed by scanning electron microscope. The minimum critical transition temperature and drug release behavior of hydrogels were investigated by ultraviolet spectrophotometry. The tetrazolium-based colorimetric assay (MTT assay) was used to investigate the toxicity and proliferation effects of PTH (1-34)-loaded thermo-sensitive hydrogel on mouse mononuclear macrophage RAW264.7 and mouse precranial osteoblasts MC3T3-E1. The effect of PTH (1-34)-loaded thermo-sensitive hydrogel on the differentiation of RAW264.7 was investigated by the tartrate-resistant acid phosphatase assay. The results showed that the PTH (1-34)-loaded thermo-sensitive hydrogel prepared in this study displayed regular three-dimensional honeycomb structure, and had good stability, thermo-sensitivity and sustained and controlled release properties, which could promote the proliferation of MC3T3-E1 cells more effectively and inhibit the differentiation of RAW264.7 into osteoclasts.
