The antitussive and expectorant mechanism of Platycodon total saponins based on metabonomics
10.16438/j.0513-4870.2021-0719
- VernacularTitle:基于代谢组学的桔梗总皂苷镇咳祛痰作用机制研究
- Author:
Xin-hong WANG
1
;
Chi ZHANG
1
;
Li ZHOU
1
;
Jin-xiang ZENG
1
;
Ling-ling REN
1
;
Zhu MAO
1
;
En YUAN
2
;
Li-fen ZHOU
2
Author Information
1. Research Center of Traditional Chinese Medicine Resources and Ethnic Minority Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, China
2. Large Precision Instrument Sharing Service Center, Jiangxi University of Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
italic>Platycodon total saponin;
antitussive;
metabonomics;
mechanism
- From:
Acta Pharmaceutica Sinica
2022;57(3):757-765
- CountryChina
- Language:Chinese
-
Abstract:
UHPLC-Q-TOF/MS metabonomics technology was used to clarify the metabolic regulation pathways by which Platycodon total saponins (PTS) exert antitussive and expectorant effects in a mouse cough model, in which coughing is induced by concentrated ammonia, and in a phenol red excretion model. After approval by the Experimental Animal Ethics Committee of Jiangxi University of Chinese Medicine (Approval No. JZLLSC-20190235), the mice were randomly divided into a normal group, a model group, a positive drug group and a PTS group. Endogenous metabolites in mouse serum were identified by UHPLC-Q-TOF/MS. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used for multivariate analysis. Metabolic pathways were analyzed by the Metaboanalyst platform. The results show that PTS can significantly prolong the cough latent period and cough frequency of mice, and significantly increase phenol red excretion. UHPLC-Q-TOF/MS identified 19 metabolites related to cough, and PTS significantly decreased 16 of them; 17 metabolites related to expectoration were identified, and PTS decreased the levels of all. Metabolic pathway analysis showed that linoleic acid metabolism, arachidonic acid metabolism and glycerophospholipid metabolism were the main pathways involved in serum metabolite changes in this mouse cough model. Linoleic acid metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, arachidonic acid metabolism, phenylalanine metabolism and α-linolenic acid metabolism were the main pathways involved in serum metabolite changes in the phenol red excretion model. This study is the first to elucidate the regulation of antitussive and expectorant metabolic pathways and the effect of PTS on these pathways.
