Bruceine D inhibits HIF-1α-mediated glucose metabolism in hepatocellular carcinoma by blocking ICAT/β-catenin interaction

Rui Huang; Lijun Zhang; Jinmei Jin; Yudong Zhou; Hongwei Zhang; Chao LV; Dong LU; Ye WU; Hong Zhang; Sanhong Liu; Hongzhuan Chen; Xin Luan; Weidong Zhang

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Bruceine D inhibits HIF-1α-mediated glucose metabolism in hepatocellular carcinoma by blocking ICAT/β-catenin interaction

Author: Rui HUANG ¹ ; Lijun ZHANG ¹ ; Jinmei JIN ¹ ; Yudong ZHOU ¹ ; Hongwei ZHANG ¹ ; Chao LV ¹ ; Dong LU ¹ ; Ye WU ¹ ; Hong ZHANG ¹ ; Sanhong LIU ¹ ; Hongzhuan CHEN ¹ ; Xin LUAN ¹ ; Weidong ZHANG ¹
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1. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Publication Type:Journal Article
Keywords: BD, bruceine D; Bruceine D; CETSA, cellular thermal shift assay; Cyt c, cytochrome c; DARTS, drug affinity responsive target stability; HCC, hepatocellular carcinoma; HIF-1α; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; Hepatocellular carcinoma; Hypoxia; ICAT; ICAT, inhibitor of β-catenin and T-cell factor; MST, microscale thermophoresis; Metabolism; ROS, reactive oxygen species; Tumor microenvironment; β-Catenin
From: Acta Pharmaceutica Sinica B 2021;11(11):3481-3492
CountryChina
Language:English
Abstract: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths, characterized by highly hypoxic tumor microenvironment. Hypoxia-inducible factor-1α (HIF-1α) is a major regulator involved in cellular response to changes of oxygen levels, supporting the adaptation of tumor cells to hypoxia. Bruceine D (BD) is an isolated natural quassinoid with multiple anti-cancer effects. Here, we identified BD could significantly inhibit the HIF-1α expression and its subsequently mediated HCC cell metabolism. Using biophysical proteomics approaches, we identified inhibitor of β-catenin and T-cell factor (ICAT) as the functional target of BD. By targeting ICAT, BD disrupted the interaction of β-catenin and ICAT, and promoted β-catenin degradation, which in turn induced the decrease of HIF-1α expression. Furthermore, BD could inhibit HCC cells proliferation and tumor growth in vivo, and knockdown of ICAT substantially increased resistance to BD treatment in vitro. Our data highlight the potential of BD as a modulator of β-catenin/HIF-1α axis mediated HCC metabolism.