Development of an eco-friendly and fast HPLC method for quantitative analysis of four nucleosides in Cordyceps and related products.
10.1016/S1875-5364(22)60162-6
- Author:
Zheng-Ming QIAN
1
,
2
;
Zi WU
3
;
Qi HUANG
3
;
Chuan-Xi WANG
4
;
Guo-Yin TAN
3
;
Wen-Jia LI
3
;
Wan-Yang SUN
4
;
Gen-Pin LV
5
;
Hao GAO
6
Author Information
1. Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China
2. Ruyuan HEC Pharm Co., Ltd., Shaoguan 512700, China.
3. Ruyuan HEC Pharm Co., Ltd., Shaoguan 512700, China.
4. Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
5. Ruyuan HEC Pharm Co., Ltd., Shaoguan 512700, China. Electronic address: dyglee@163.com.
6. Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: tghao@jnu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Adenosine;
Cordyceps;
Guanosine;
Inosine;
Uridine
- MeSH:
Adenosine;
Chromatography, High Pressure Liquid;
Cordyceps;
Nucleosides
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(12):954-960
- CountryChina
- Language:English
-
Abstract:
An eco-friendly and fast HPLC method was developed for the determination of adenosine, inosine, guanosine and uridine in Cordyceps and related products (fermented mycelia of Hirsutella sinensis andPaecilomyces hepiali). The sample was ultrasonically extracted using 0.5% phosphoric acid solutions for 2.5 min. Sample separation was performed on a Poroshell SB-Aq column (50 mm × 4.6 mm, 2.7 μm) using eco-friendly mobile phase consisting of formic acid and ammonium formate aqueous solution at a flow rate of 1.0 mL·min
