Protective effect of Pai-Nong-San against AOM/DSS-induced CAC in mice through inhibiting the Wnt signaling pathway.
10.1016/S1875-5364(22)60143-2
- Author:
Meng-Meng ZHANG
1
;
Deng-Ke YIN
2
,
3
,
4
,
5
;
Xue-Lin RUI
1
;
Fu-Ping SHAO
1
;
Jia-Cheng LI
1
;
Li XU
1
;
Ye YANG
2
,
4
,
6
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China.
2. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China
3. Anhui Provincial Key Laboratory for Chinese Medicinal Formula, Hefei 230031, China
4. Institute of Pharmaceutics, Anhui Academy of Chinese Medicine, Hefei 230021, China
5. Anhui Provincial Key Laboratory of Research & Development of Chinese Medicine, Hefei 230021, China. Electronic address: yindengke@ahtcm.edu.cn.
6. Anhui Provincial Key Laboratory of Research & Development of Chinese Medicine, Hefei 230021, China. Electronic address: Y.Yang@ahtcm.edu.cn.
- Publication Type:Journal Article
- Keywords:
AOM-DSS;
CAC;
Gut microbiota;
Pai-Nong-San;
Wnt signaling pathway
- MeSH:
Animals;
Azoxymethane/toxicity*;
CD8-Positive T-Lymphocytes;
Colitis/genetics*;
Dextran Sulfate/toxicity*;
Disease Models, Animal;
Drugs, Chinese Herbal/pharmacology*;
Glycogen Synthase Kinase 3 beta;
Mice;
Mice, Inbred C57BL;
RNA, Ribosomal, 16S;
Wnt Signaling Pathway/drug effects*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(12):912-920
- CountryChina
- Language:English
-
Abstract:
Pai-Nong-San (PNS), a prescription of traditional Chinese medicine, has been used for years to treat abscessation-induced diseases including colitis and colorectal cancer. This study was aimed to investigate the preventive effects and possible protective mechanism of PNS on a colitis-associated colorectal cancer (CAC) mouse model induced by azoxymethane (AOM)/dextran sodium sulfate (DSS). The macroscopic and histopathologic examinations of colon injury and DAI score were observed. The inflammatory indicators of intestinal immunity were determined by immunohistochemistry and immunofluorescence. The high throughput 16S rRNA sequence of gut microbiota in the feces of mice was performed. Western blot was used to investigate the protein expression of the Wnt signaling pathway in colon tissues. PNS improved colon injury, as manifested by the alleviation of hematochezia, decreased DAI score, increased colon length, and reversal of pathological changes. PNS treatment protected against AOM/DSS-induced colon inflammation by regulating the expression of CD4
