A target lipidomics approach to investigate the acute inflammatory irritation induced by indolealkylamines from Chansu water fraction in rats.
10.1016/S1875-5364(21)60117-6
- Author:
Xin YANG
1
;
Wu-Yue CHEN
1
;
Yan GONG
1
;
Liu-Qing DI
2
;
Jin-Ao DUAN
1
;
Jing ZHOU
3
;
Hong-Yue MA
4
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2. Jiangsu Engineering Research Center for Efficient Delivery System of TCM, Nanjing University of Chinese Medicine, Nanjing 210023, China.
3. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: zhoujing_nj@126.com.
4. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: hongyuema@njucm.edu.cn.
- Publication Type:Journal Article
- Keywords:
Acute inflammatory irritation;
Chansu;
Eicosanoids;
Lipidomics;
Molecular docking
- MeSH:
Animals;
Bufanolides;
Edema/drug therapy*;
Inflammation;
Lipidomics;
Molecular Docking Simulation;
Rats;
Water
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(11):856-867
- CountryChina
- Language:English
-
Abstract:
Chansu has demonstrated adverse reactions in clinical settings, which is associated with its toxicity and limits its clinical applications. But there are methodological limitations for drug safety evaluation. In the current study, ultra-high performance liquid chromatography, lipidomic profiling, and molecular docking were used to systemically assess Chansu-induced acute inflammatory irritation and further identify the underlying drug targets. Compared with the EtOAc extract, Chansu water fraction containing indolealkylamines caused acute inflammatory irritation in rats, including acute pain (spontaneous raising foot reaction), and inflammation (paw edema). At the molecular level, lipids analysis revealed significantly higher levels of pro-inflammatory mediators of the COX and LOX pathways. However, anti-inflammatory mediators from the CYP 450, ALA, and DHA pathways markedly decreased after exposure to Chansu water fraction. Moreover, four indolealkylamines from Chansu showed a high theoretical affinity to a known irritation target, 5-HT
