Resveratrol alleviates Kawasaki disease-induced myocardial injury via inhibition of apoptosis and autophagy.
10.11817/j.issn.1672-7347.2021.200843
- Author:
Fengmei XIONG
1
;
Ruiping LIU
2
;
Huanli GUO
3
;
Dongmei WU
3
;
Na SUN
4
Author Information
1. Department of Pharmacy, Xi'an Children's Hospital, Xi'an 710003. xiongfeng10@126.com.
2. Department of Nutrition, Xi'an Children's Hospital, Xi'an 710003.
3. Department of Pharmacy, Xi'an Children's Hospital, Xi'an 710003.
4. Institute of Basic Medical Science, Xi'an Medical University, Xi'an 710021, China. 651665992@qq.com.
- Publication Type:Journal Article
- Keywords:
Kawasaki disease;
apoptosis;
autophagy;
resveratrol
- MeSH:
Animals;
Apoptosis;
Autophagy;
Male;
Mucocutaneous Lymph Node Syndrome/drug therapy*;
Rats;
Rats, Sprague-Dawley;
Resveratrol/therapeutic use*;
Stroke Volume;
Ventricular Function, Left
- From:
Journal of Central South University(Medical Sciences)
2021;46(10):1102-1108
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To explore the effect of resveratrol (Res) on Kawasaki disease (KD)-induced myocardial injury and to evaluate its effect on apoptosis and autophagy.
METHODS:Forty-eight juvenile male Sprague Dawley rats were randomly divided into a control group, a Res group, a lactobacillus casei cell wall extract (LCWE)-induced Kawasaki disease group (KD group), and a LCWE-induced Kawasaki disease + Res treatment group (Res+KD group). The control group was intraperitoneally injected with saline. The Res group was intraperitoneally injected with resveratrol (100 mg/kg). The KD group was intraperitoneally injected with 0.5 mL LCWE (1 mg/mL). The Res+KD group was intraperitoneally injected with 0.5 mL LCWE (1 mg/mL) and resveratrol (100 mg/kg). After 4 weeks, the left ventricular ejection fraction (LVEF) and short axis shortening rate (LVFS) were detected by echocardiography. The apoptotic rate was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) staining. The levels of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), microtubule-associated protein 1 light chain 3β (LC3B), Beclin-1, autophagy related 5 (Atg5) and sequestosome-1 (p62) were detected by Western blotting. The formation of autophagosome was observed under transmission electron microscope.
RESULTS:There was no significant difference in the above-mentioned indexes between the control group and the Res group (all
CONCLUSIONS:Res can attenuate the KD-induced myocardial injury via inhibiting the apoptosis and autophagy.
