Differential expression of circular RNA in patients with white matter hyperintensity and cognitive impairment.
10.11817/j.issn.1672-7347.2021.200692
- Author:
Xuewei DONG
1
;
Hongying SUN
2
;
Jian MAO
3
;
Shuya ZHANG
3
;
Chenxi MENG
3
Author Information
1. Department of Neurology, First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou Inner Mongolia 014010, China. 2461024598@qq.com.
2. Department of Neurology, First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou Inner Mongolia 014010, China. sunhongying2004@sina.com.
3. Department of Neurology, First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou Inner Mongolia 014010, China.
- Publication Type:Journal Article
- Keywords:
circular RNA;
cognitive impairment;
differential expression;
target gene prediction;
white matter hyperintensity
- MeSH:
Cognitive Dysfunction/genetics*;
Female;
Humans;
Leukocytes, Mononuclear;
Male;
MicroRNAs;
RNA/genetics*;
RNA, Circular;
Software;
White Matter
- From:
Journal of Central South University(Medical Sciences)
2021;46(10):1080-1089
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:White matter hyperintensity (WMH) is an important factor leading to cognitive impairment, and the mechanism has not been clarified. In recent years, studies have found that circular RNA (circRNA) has differential expression in cerebrovascular diseases. This study aims to analyze the expression profile of circRNA in peripheral blood mononuclear cell (PBMC) of patients with WMH with cognitive impairment, to screen the differentially expressed circRNA, and to explore the possible role of circRNA in WMH with cognitive impairment.
METHODS:CircRNA microarray was used to detect the circRNA expression profile of PBMC in patients with WMH with cognitive impairment, and in patients with WMH without cognitive impairment as well as in normal controls (3 cases each, male to female ratio of 2꞉1). The differentially expressed circRNA in patients with WMH with cognitive impairment was screened. The screening criteria for differentially expressed circRNA was fold change (FC) ≥2.0 (|log
RESULTS:Compared with the control group, there were 5 significantly up-regulated circRNA and 3 down-regulated circRNA in the WMH with cognitive impairment group; 8 circRNA were significantly up-regulated and 2 were down-regulated in the WMH without cognitive impairment group. When compared with the WMH with cognitive impairment group, no co-differentially expressed circRNA was found in WMH without cognitive impairment group and control group. Compared with the control group, the expression of hsa_circ_0092222 was up-regulated and the expressions of hsa_circ_0000662 and hsa_circ_0083773 were down-regulated in the WMH with cognitive impairment group and the WMH without cognitive impairment group, and there was no significant difference between the 2 groups (all
CONCLUSIONS:The circRNA expression profile of patients with WMH is changed significantly. The differentially expressed circRNA may be the cause of WMH; Hsa_circ_0092222, hsa_circ_0000662, and hsa_circ_0083773 may regulate the expression of target genes by targeting adsorption of the target miRNA, leading to brain white matter damage through Janus kinase 2 (JAK2)/signal transducers and activators of transcription (STAT3) signal pathway and Wnt signal pathway.There is no significant difference in circRNA expression profile between WMH with or without cognitive impairment. Cognitive impairment in patients with WMH may have other reasons.
