Impact of microRNA
10.11817/j.issn.1672-7347.2021.200764
- Author:
Miaomiao LIU
1
;
Haoyu DENG
2
;
Yajie ZHAO
2
;
Can LI
2
;
Hua LIU
3
Author Information
1. Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. liumm0527@163.com.
2. Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
3. Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. liuhua23@csu.edu.cn.
- Publication Type:Journal Article
- Keywords:
microRNA-21-5p;
recombinant sclerostin domain containing protein 1;
thyroid cancer
- MeSH:
Adaptor Proteins, Signal Transducing;
Apoptosis/genetics*;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Humans;
MicroRNAs/genetics*;
Phosphatidylinositol 3-Kinases/metabolism*;
Proto-Oncogene Proteins c-akt/metabolism*;
Thyroid Neoplasms/genetics*
- From:
Journal of Central South University(Medical Sciences)
2021;46(10):1054-1062
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To explore the molecular mechanism for thyroid cancer metastasis via analyzing the role of microRNA (miR)-21-5p and its target gene recombinant sclerostin domain containing protein 1 (SOSTDC1) in thyroid cancer.
METHODS:The target miR-21-5p was screened through bioinformatics analysis and cell verification, and the thyroid cancer cell lines was transfected with miR-21-5p inhibitor. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test, flow cytometry, and cell scratch test were used to detect the proliferation, apoptosis and migration of thyroid cancer cells in the miR-21-5p inhibitor group and the inhibitor control group, respectively. The luciferase report experiment was used to verify the relationship between miR-21-5p and SOSTDC1, Western blotting was used to detect the expression levels and phosphorylation levels of SOSTDC1,phosphatidylinositol 3 kinase (PI3K), protein kinase B (Akt) and mitogen-activated protein kinases (MAPK), extracellular regulated protein kinases (ERK) in thyroid cancer cells.
RESULTS:MiR-21-5p was significantly increased in thyroid cancer cells,which was negatively correlated with SOSTDC1 (
CONCLUSIONS:MiR-21-5p in thyroid cancer cells can target the expression of SOSTDC1 and affect the activities of PI3K/Akt and MAPK/ERK, thereby inhibiting the apoptosis of thyroid cancer cells and promoting cell proliferation and migration.
