Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics.
10.1007/s11655-021-3337-2
- Author:
Xian-Pei HENG
1
;
Zhi-Ta WANG
2
;
Liang LI
2
;
Liu-Qing YANG
2
;
Su-Ping HUANG
3
Author Information
1. Department of Endocrinology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, 350004, China. hengxianpei@hotmail.com.
2. Department of Endocrinology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, 350004, China.
3. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Dangua Recipe;
Nampt;
glycolipid metabolism;
therapeutic target;
transcriptomics
- MeSH:
Animals;
Diabetes Mellitus, Experimental;
Drugs, Chinese Herbal;
Glycolipids;
Liver;
Metabolic Diseases;
Rats;
Rats, Sprague-Dawley;
Transcriptome/genetics*
- From:
Chinese journal of integrative medicine
2022;28(2):130-137
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the mechanisms of Dangua Recipe (DGR) in improving glycolipid metabolism based on transcriptomics.
METHODS:Sprague-Dawley rats with normal glucose level were divided into 3 groups according to a random number table, including a conventional diet group (Group A), a DGR group (Group B, high-calorie diet + 20.5 g DGR), and a high-calorie fodder model group (Group C). After 12 weeks of intervention, the liver tissue of rats was taken. Gene sequence and transcriptional analysis were performed to identify the key genes related to glycolipid metabolism reflecting DGR efficacy, and then gene or protein validation of liver tissue were performed. Nicotinamide phosphoribosyl transferase (Nampt) and phosphoenolpyruvate carboxykinase (PEPCK) proteins in liver tissues were detected by enzyme linked immunosorbent assay, fatty acid synthase (FASN) protein was detected by Western blot, and fatty acid binding protein 5 (FABP5)-mRNA was detected by quantitative real-time polymerase chain reaction. Furthermore, the functional verification was performed on the diabetic model rats by Nampt blocker (GEN-617) injected in vivo. Hemoglobin A
RESULTS:Totally, 257 differential-dominant genes of Group A vs. Group C and 392 differential-dominant genes of Group B vs. Group C were found. Moreover, 11 Gene Ontology molecular function terms and 7 Kyoto Encyclopedia of Genes and Genomes enrichment pathways owned by both Group A vs. Group C and Group C vs. Group B were confirmed. The liver tissue target validation showed that Nampt, FASN, PEPCK protein and FABP5-mRNA had the same changes consistent with transcriptome. The in vivo functional tests showed that GEN-617 increased body weight, HbA
CONCLUSION:Nampt activation was one of the mechanisms about DGR regulating glycolipid metabolism.
