Urinary donor-derived cell-free DNA as a non-invasive biomarker for BK polyomavirus-associated nephropathy.
- Author:
Jia SHEN
1
;
Luying GUO
1
;
Wenhua LEI
1
;
Shuaihui LIU
1
;
Pengpeng YAN
1
;
Haitao LIU
2
;
Jingyi ZHOU
1
;
Qin ZHOU
1
;
Feng LIU
2
;
Tingya JIANG
2
;
Huiping WANG
1
;
Jianyong WU
1
;
Jianghua CHEN
1
;
Rending WANG
3
Author Information
- Publication Type:Journal Article
- Keywords: BK polyomavirus-associated nephropathy (BKPyVAN); Differential diagnosis; Donor-derived cell-free DNA (ddcfDNA); T cell-mediated rejection (TCMR); Urine
- From: Journal of Zhejiang University. Science. B 2021;22(11):917-928
- CountryChina
- Language:English
- Abstract: BK polyomavirus-associated nephropathy (BKPyVAN) is a common cause of allograft failure. However, differentiation between BKPyVAN and type I T cell-mediated rejection (TCMR) is challenging when simian virus 40 (SV40) staining is negative, because of the similarities in histopathology. This study investigated whether donor-derived cell-free DNA (ddcfDNA) can be used to differentiate BKPyVAN. Target region capture sequencing was applied to detect the ddcfDNAs of 12 recipients with stable graft function, 22 with type I TCMR, 21 with proven BKPyVAN, and 5 with possible PyVAN. We found that urinary ddcfDNA levels were upregulated in recipients with graft injury, whereas plasma ddcfDNA levels were comparable for all groups. The median urinary concentrations and fractions of ddcfDNA in proven BKPyVAN recipients were significantly higher than those in type I TCMR recipients (10.4 vs. 6.1 ng/mL,
