Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats.

Zhinan Zhou; Xiang Chen; Min Zhu; Weiwei Wang; Zheng AO; Jiafu Zhao; Wen Tang; Lei Hong

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Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats.

Author: Zhinan ZHOU ¹ ; Xiang CHEN ² ; Min ZHU ¹ ; Weiwei WANG ¹ ; Zheng AO ¹ ; Jiafu ZHAO ¹ ; Wen TANG ¹ ; Lei HONG ¹
Author Information

1. Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, College of Animal Science, Guizhou University, Guiyang 550025, China.
2. Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, College of Animal Science, Guizhou University, Guiyang 550025, China. XChen2@gzu.edu.cn.
Publication Type:Journal Article
Keywords: Cathepsin D (CTSD); Cell apoptosis; Cell cycle; Cell proliferation; Granulosa cells; Litter size trait
From: Journal of Zhejiang University. Science. B 2021;22(11):893-905
CountryChina
Language:English
Abstract: Cathepsin D (CTSD), the major lysosomal aspartic protease that is widely expressed in different tissues, potentially regulates the biological behaviors of various cells. Follicular granulosa cells are responsive to the increase of ovulation number, hence indirectly influencing litter size. However, the mechanism underlying the effect of CTSD on the behaviors of goat granulosa cells has not been fully elucidated. This study used immunohistochemistry to analyze CTSD localization in goat ovarian tissues. Moreover, western blotting was applied to examine the differential expression of CTSD in the ovarian tissues of monotocous and polytocous goats. Subsequently, the effects of CTSD knockdown on cell proliferation, apoptosis, cell cycle, and the expression of candidate genes of the prolific traits, including bone morphogenetic protein receptor IB (