Generation of developmentally competent oocytes and fertile mice from parthenogenetic embryonic stem cells.
10.1007/s13238-021-00865-4
- Author:
Chenglei TIAN
1
;
Linlin LIU
1
;
Ming ZENG
2
;
Xiaoyan SHENG
1
;
Dai HENG
1
;
Lingling WANG
2
;
Xiaoying YE
1
;
David L KEEFE
3
;
Lin LIU
4
Author Information
1. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China.
2. Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, Tianjin, 300071, China.
3. Department of Obstetrics and Gynecology, NYU Langone Health, 550 First Avenue, New York, NY, 10012, USA.
4. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. liulin@nankai.edu.cn.
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords:
imprinting;
meiosis;
oocytes;
parthenogenetic embryonic stem cells;
primordial germ cell-like cells
- MeSH:
Animals;
Female;
Mice;
Mice, Transgenic;
Mouse Embryonic Stem Cells/metabolism*;
Oocytes/metabolism*;
Parthenogenesis
- From:
Protein & Cell
2021;12(12):947-964
- CountryChina
- Language:English
-
Abstract:
Parthenogenetic embryos, created by activation and diploidization of oocytes, arrest at mid-gestation for defective paternal imprints, which impair placental development. Also, viable offspring has not been obtained without genetic manipulation from parthenogenetic embryonic stem cells (pESCs) derived from parthenogenetic embryos, presumably attributable to their aberrant imprinting. We show that an unlimited number of oocytes can be derived from pESCs and produce healthy offspring. Moreover, normal expression of imprinted genes is found in the germ cells and the mice. pESCs exhibited imprinting consistent with exclusively maternal lineage, and higher X-chromosome activation compared to female ESCs derived from the same mouse genetic background. pESCs differentiated into primordial germ cell-like cells (PGCLCs) and formed oocytes following in vivo transplantation into kidney capsule that produced fertile pups and reconstituted ovarian endocrine function. The transcriptome and methylation of imprinted and X-linked genes in pESC-PGCLCs closely resembled those of in vivo produced PGCs, consistent with efficient reprogramming of methylation and genomic imprinting. These results demonstrate that amplification of germ cells through parthenogenesis faithfully maintains maternal imprinting, offering a promising route for deriving functional oocytes and having potential in rebuilding ovarian endocrine function.
