From bulk, single-cell to spatial RNA sequencing.
10.1038/s41368-021-00146-0
- Author:
Xinmin LI
1
;
Cun-Yu WANG
2
Author Information
1. UCLA Technology Center for Genomics & Bioinformatics, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. xinminli@mednet.ucla.edu.
2. Laboratory of Molecular Signaling, Division of Oral Biology and Medicine, School of Dentistry and Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA, USA. cwang@dentistry.ucla.edu.
- Publication Type:Review
- MeSH:
Humans;
Neoplasms;
Precision Medicine;
Sequence Analysis, RNA;
Whole Exome Sequencing
- From:
International Journal of Oral Science
2021;13(1):36-36
- CountryChina
- Language:English
-
Abstract:
RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNAseq), popular single cell RNA sequencing (scRNAseq) to newly emerged spatial RNA sequencing (spRNAseq). Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA activities spatially, representing next generation of RNA sequencing. This article highlights these technologies, characteristic features and suitable applications in precision oncology.
