Inhibitory Effect of Ascorbic Acid on Myelodysplastic Syndrome Cells and Its Mechanism.
10.19746/j.cnki.issn.1009-2137.2021.06.027
- Author:
Hai-Yue NIU
1
;
Zong-Hong SHAO
1
;
Hua-Quan WANG
2
Author Information
1. Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
2. Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China E-mail: wanghuaquan@tmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Ascorbic Acid;
Cell Line, Tumor;
Cell Proliferation;
Decitabine;
Humans;
Myelodysplastic Syndromes
- From:
Journal of Experimental Hematology
2021;29(6):1851-1857
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibitory effect of ascorbic acid single or combination of decitabine on tumor cells of myelodysplastic syndrome (MDS) and explore its related mechanism.
METHODS:The human MDS cell lines SKM-1 and MUTZ-1 were treated with different concentrations of ascorbic acid, and the cell proliferation activity was detected by the CCK-8 assay. The reactive oxygen species (ROS) level, labile iron pool (LIP), cell cycle, and apoptosis of SKM-1 and MUTZ-1 cells were detected by flow cytometry. The control group, ascorbic acid monotherapy group, decitabine monotherapy group, and combination group of ascorbic acid and decitabine were set up, the cell proliferation activity and apoptosis were detected in each group.
RESULTS:High-dose ascorbic acid could reduce the cell proliferation activity of SKM-1 (R=0.886, p=0.000) and MUTZ-1 (R=0.880, p=0.000). With the increase of ascorbic acid concentration, the ROS level in SKM-1 and MUTZ-1 cells increased (r=0.816, r=0.942), the proportion of cells stagnation in G
CONCLUSION:High-dose ascorbic acid shows a cytotoxic effect on MDS tumor cells, inhibiting cell proliferation and increasing apoptosis. Ascorbic acid combined decitabine have a synergistic effect of anti-MDS tumor cells.
