RBM38 Mediates the Proliferation of Acute Myeloid Leukemia Cells HL-60 by Regulating FZD1 mRNA Stability.
10.19746/j.cnki.issn.1009-2137.2021.06.014
- Author:
Ya-Peng ZHANG
1
;
Da GAO
1
;
Peng WU
2
Author Information
1. Department of Hematology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, Inner Mongolia Autonomous Region, China.
2. Department of Hematology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, Inner Mongolia Autonomous Region, China E-mail: 408253802@qq.com.
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
Frizzled Receptors;
HL-60 Cells;
Humans;
Leukemia, Myeloid, Acute;
RNA Stability;
RNA-Binding Proteins/metabolism*
- From:
Journal of Experimental Hematology
2021;29(6):1775-1779
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the regulatory function of RNA binding motif protein 38 (RBM38) in human acute myeloid leukemia cells HL-60 and its mechanism.
METHODS:The lentivirus carriers of overexpressed and knockdown RBM38 were constructed. After HL-60 cells were transfected, Western blot was used to analyze the expression level of RBM38 in HL-60 cells. The cell proliferation and cycle of HL-60 were detected by CCK-8 assay and flow cytometry assay, respectively. RNA immunoprecipitation coupled real-time PCR (RIP-qPCR) was used to detect the combination of RBM38 with mRNAs. Actinomycin D treatment followed by real-time PCR (AcD-qPCR) was used to detect the effect of RBM38 on the stability of target mRNAs.
RESULTS:RBM38 in HL-60 cells was overexpressed or inhibited by lentivirus transduction. Overexpressed RBM38 promoted the cell cycle and proliferation of HL-60, while RBM38 knockdown repressed the two processes. RBM38 showed an interaction with FZD1 mRNA and enhancement of its stability.
CONCLUSION:RBM38 can regulate cell proliferation of HL-60 by improving the stability of FZD1 mRNA.
