Transcriptome Analysis of Chronic Myelogenous Leukemia Cell Line with Imatinib Resistance.
10.19746/j.cnki.issn.1009-2137.2021.06.004
- Author:
Xiao HAN
1
;
Zhi-Kui DENG
2
;
Cheng-Wan ZHANG
1
;
Liang YU
2
;
Xiao-Ning LIU
3
Author Information
1. Department of Central Laboratory, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
2. Department of Hematology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
3. Department of Hematology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China,E-mail:hayylxn@163.com.
- Publication Type:Journal Article
- MeSH:
Drug Resistance, Neoplasm;
Gene Expression Profiling;
Humans;
Imatinib Mesylate/pharmacology*;
K562 Cells;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics*
- From:
Journal of Experimental Hematology
2021;29(6):1714-1718
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the regulation of chronic myelogenous leukemia （CML） imatinib resistant genes, in order to improve the therapeutic effect of CML imatinib resistant patients.
METHODS:The human CML cell line K562 and imatinib-resistant K562 cells (K562/G01) were collected, and transcriptome of the cells were achieved by RNA-seq. The sequencing data were analyzed by using standard procedures.
RESULTS:Compared with K562 cells, 464 genes were significantly changed in K562/G01 cells, including 163 up-regulated and 301 down-regulated genes. The GO function annotation analysis and KEGG pathway analysis results showed that the differentially expressed genes were mainly involved in biological processes such as oxidative phosphorylation, localization to protein organelle, ribonucleoprotein complex biogenesis and so on. Gene Set Enrichment Analysis (GSEA) plots showed that 5 gene-sets were up-regulated in K562/G01 significantly, including the pathway of TGF-beta, mTOR and CML.
CONCLUSION:CML imatinib resistance is associated with oxidative phosphorylation, during which the pathway of TGF-beta and mTOR are significantly up-regulated.
