The Effect of MicroRNA-143 on the Proliferation and Apoptosis of Leukemia Cell Line U-937.
10.19746/j.cnki.issn.1009-2137.2021.06.001
- Author:
Jin-Long HUANG
1
;
Jing-Ping FANG
2
;
Jian-Zhen SHEN
3
Author Information
1. Department of Hematology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China.
2. College of Life Science, Fujian Normal University, Fuzhou 350117, Fujian Province, China.
3. Department of Hematology, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China,E-mail: doctorjzs@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line;
Cell Proliferation;
Humans;
Leukemia, Myeloid, Acute/genetics*;
MicroRNAs/genetics*
- From:
Journal of Experimental Hematology
2021;29(6):1695-1703
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of microRNA-143 (miR-143) in patients with acute myeloid leukemia (AML), and the effect of miR-143 over-expression on the proliferation and apoptosis of AML cells. And to verify the targeting relationship between miR-143 and long non-coding RNA MALAT-1. So as to explore the possible regulatory mechanism of miR-143 in the pathogenesis of AML.
METHODS:The expression of miR-143 in bone marrow cells of AML patients was detected by RT-qPCR. After miR-143 was over-expressed in U-937 cell lines, the proliferation of U-937 cell lines was detected by CCK-8, clone formation assay, and flow cytometry. In addition, cell apoptosis was detected by Hoechst 33258 fluorescence staining and flow cytometry. At the same time, bioinformatics, RT-qPCR and dual luciferase reporter gene assay were used to predicted and verified the targeting relationship between miR-143 and MALAT-1.
RESULTS:Expression of miR-143 in AML patients was significantly lower than those in normal controls. Over-expressed miR-143 could inhibit the proliferation of U-937 cells and promote the apoptosis of the cell. The miR-143 binding site was located on the MALAT-1 RNA sequence, and MALAT-1 was down-regulated in U-937 cells after over-expressed miR-143. However, the expression of miR-143 showed no significantly changed after MALAT-1 silencing.
CONCLUSION:Expression of miR-143 in AML patients is lower than that in normal controls. Over-expression of miR-143 can inhibit the proliferation of U-937 cells and promote its apoptosis. And its mechanism may be related to its targe regulation of MALAT-1.
