Antimicrobial Effects of Oleanolic Acid against Streptococcus mutans and Streptococcus sobrinus Isolated from a Korean Population.
- Author:
Min Jung KIM
1
;
Chun Sung KIM
;
Woo Hyung HA
;
Byung Hoon KIM
;
Yun Kyong LIM
;
Soon Nang PARK
;
Yu Jin CHO
;
Myungmi KIM
;
Jang Hyuk KO
;
Soon Sung KWON
;
Yeong Mu KO
;
Joong Ki KOOK
Author Information
1. Department of Oral Biochemistry, School of Dentistry, Chosun University, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759 Korea. jkkook@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Antimicrobial effect;
oleanolic acid;
Streptococcus mutans
- MeSH:
Bacteria;
Dental Caries;
Fibroblasts;
Humans;
KB Cells;
Oleanolic Acid;
Oral Hygiene;
Periodontal Ligament;
Plants, Medicinal;
Streptococcus;
Streptococcus mutans;
Streptococcus sobrinus
- From:International Journal of Oral Biology
2010;35(4):191-195
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Oleanolic acid is a natural triterpenoid that exists widely in foods and some medicinal herbs. The purpose of this study was to determine the antimicrobial activity of oleanolic acid against Streptococcus mutans strains isolated from a Korean population. Antimicrobial activity against these bacteria was evaluated by minimal inhibitory concentration (MIC) and time kill curves. The tolerance of human gingival fibroblasts and human periodontal ligaments to oleanolic acid was tested using a methyl thiazolyl tetrazolium (MTT) assay. The MIC90 value of oleanolic acid for both S. mutans and S. sobrinus isolated from Koreans was 8 microg/ml. Oleanolic acid showed bactericidal effects against S. mutans ATCC 25175T and S. sobrinus ATCC 33478T at 1 x MIC (8 microg/ml) and had no cytotoxic effects against KB cells at this dose. The results suggest that oleanolic acid could be useful in the future development of oral hygiene products for the prevention of dental caries.
