Application and Research Progress of Lineage Tracing in Differentiation Mechanism of Hematopoietic Stem Cells--Review.
10.19746/j.cnki.issn.1009-2137.2021.05.053
- Author:
Yan DONG
1
;
Fang DONG
2
;
Hai Tao BAI
1
;
Hideo EMA
3
Author Information
1. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Tianjin 300020, China,Center for Stem Cell Medicine, Chinese Academy of Medical Sciences,Tianjin 300020, China.
3. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China,Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, Tianjin 300020, China,Department of Stem Cell Regenerative Medicine, Peking Union Medical College, Tianjin 300020, China.
- Publication Type:Review
- MeSH:
Cell Differentiation;
Hematopoiesis;
Hematopoietic Stem Cells
- From:
Journal of Experimental Hematology
2021;29(5):1690-1694
- CountryChina
- Language:Chinese
-
Abstract:
Hematopoietic stem cells (HSCs) reside at the top of the hierarchy and have the ability to differentiate to variety of hematopoietic progenitor cells (HPCs) or mature hematopoietic cells in each system. At present, the procress of HSC and HPC differentiating to the complete hematopoietic system under physiological and stressed conditions is poorly understood. In vivo lineage tracing is a powerful technique that can mark the individual cells and identify the differentiation pathways of their daughter cells, it takes as a strong technical system to research HSC. Traditional lineage tracing studies mainly rely on imaging techniques with fluorescent dyes and nucleic acid analogs. Recently, newly cell tracing technologies have been invented, and the combination of clonal tracing and DNAsequencing technologies have provided a new perspective on cell state, cell fate, and lineage commitment at the single cell level. In this review, these new tracing methods were introduce and discuss, and their advantages over traditional methods in the study of hematopoiesis were summarized briefly.
