Effects of HMGB1 on Proliferation and Secretion of Human Bone Marrow Mesenchymal Stem Cells.
10.19746/j.cnki.issn.1009-2137.2021.05.041
- Author:
Shuo YANG
1
;
Hong-Yun LIU
1
;
Duo-Lan NAREN
1
;
Guo-Yang ZHANG
1
;
Xiao-Yan LIU
1
;
Peng-Feng YANG
2
;
Jie-Yu WANG
1
;
Li-Ping MA
3
Author Information
1. Department of Hematology, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
2. Department of Ultrasound，The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510145, Guangdong Province, China.
3. Department of Hematology, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China,E-mail: 18922182815@163.com.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
Cell Differentiation;
Cell Proliferation;
Cells, Cultured;
HMGB1 Protein;
Humans;
Mesenchymal Stem Cells
- From:
Journal of Experimental Hematology
2021;29(5):1631-1636
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of high mobility group protein 1(HMGB1) on the proliferation and cytokine expression of human bone marrow mesenchymal stem cells (MSC).
METHODS:Different concentrations of recombinant human HMGB1 protein (100, 200, 400, 800 and 1000 ng/ml) were incubated with MSC for 24, 48, 72 h and the proliferation of MSC were detected respectively by using the CCK-8 method and flow cytometry. The best concentrations of HMGB1 incubated with MSC was determined (200 ng/ml, 1000 ng/ml), and the flow cytomerty was used to determine the effect of HMGB1 on the proliferation of MSC. The mRNA expression levels of IL-10, TGF- β1, TSG-6 and IFN-γ in MSC incubated with HMGB1 protein were detected by real-time quantitative PCR and ELISA.
RESULTS:The result of MSC identification and flow cytometry showed that the CD105, CD73 and CD90 were expressed, but did not expression CD45, CD34, CD11b, CD19 and HLA-DR; CCK-8 showed that HMGB1 at the concentrations of 100 ng/ml, 200 ng/ml and 400 ng/ml could promote the proliferation of MSC incubated for 24, 48 and 72 h as compared with the control group (P<0.05), and the most effective concentration was 200 ng/ml; flow cytometry showed that the compared with the control group, HMGB1 200 ng/ml could induce MSC from G1 phase to S phase to promote the proliferation of MSC; QPCR showed that the mRNA expression of MSC cytokines IL-10, TGF-β1, TSG-6 increased while IFN-γ decreased at the concentration of 200 ng/ml HMGB1 as compared with the control group. ELISA experiments showed that the HMGB1 200 ng/ml acting on MSC for 48 h could significantly promoted the secretion of IL-10, TGF-β 1 and TSG-6(P<0．05), while IFN-γ showed no significant difference as compared with control group.
CONCLUSION:Recombinant human HMGB1 can promote the proliferation and secretion of MSC in healthy people.
