Enhanced Autophagy Suppresses Proplatelet Formation in Pediatric Immune Thrombocytopenia.
10.19746/j.cnki.issn.1009-2137.2021.05.032
- Author:
Qi WANG
1
;
Yang LI
1
;
Tao FENG
1
;
Xiao FENG
1
;
Zheng-Hua JI
1
;
Xue-Qiang JI
1
;
Xue-Jun SHAO
2
Author Information
1. Department of Clinical Laboratory Examination, Children's Hospital of Soochow University, Suzhou 215025, Jiangsu Province, China.
2. Department of Clinical Laboratory Examination, Children's Hospital of Soochow University, Suzhou 215025, Jiangsu Province, China,E-mail: xjshao@suda.edu.cn.
- Publication Type:Journal Article
- MeSH:
Autophagy;
Blood Platelets;
Humans;
Megakaryocytes;
Purpura, Thrombocytopenic, Idiopathic;
Thrombocytopenia
- From:
Journal of Experimental Hematology
2021;29(5):1577-1581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of enhanced autophagy in megakaryocyte to proplatelet formation in children with immune thrombocytopenia(ITP).
METHODS:Giemsa staining and immunofluorescence staining were used to observe megakaryocyte morphology and proplatelet formation, Western blot was used to determine the expression of cytoskeleton protein and autophagy related protein. Autophagr regulation drugs Rap or 3-MA was used to regulate autophagy of megakaryocytes.
RESULTS:Some vacuole-like structures was found in ITP megakaryocytes of the children, the expression of LC3II/I (ITP 1.32±0.18； Ctrl 0.49±0.16，P<0.05) and Atg5-Atg12 (ITP 0.69±0.17； Ctrl 0.12±0.08，P<0.05) was significantly higher in ITP children as compared with those in control group. The immu- nofluorescence staining showed that the cytoskeleton arrangement in megakaryocytes of ITP children was abnormal, and the phosphorylation of myosin light chain was also increased(ITP 0.74±0.09, Ctrl 0.05±0.02，P<0.05). In vitro, inducer or inhibitor of autophagy could regulate the production of proplatelet and the expression of cell cycle related protein, including CyclinD1（Veh 1.08±0.12; Rap 0.46±0.04; Rap+3-MA 0.70±0.03）, CyclinD2（Veh 0.47±0.04; Rap 0.27±0.04; Rap+3-MA 0.41±0.03）, P21（Veh 0.15±0.01; Rap 0.04±0.01; Rap+3-MA 0.05±0.01）.
CONCLUSION:Enhanced autophagy is the key factor of poor proplatelet formation in megakaryocytes of ITP children.
