Regulation of TRIP13 on Proliferation and Apoptosis of B-Cell Lymphoma Cells and Its Mechanism.
10.19746/j.cnki.issn.1009-2137.2021.05.017
- Author:
Su-Ran YAN
1
;
Qing ZHANG
1
;
Xing-Chen LIU
1
;
Jing-Jing YUAN
1
;
Fan ZHANG
1
;
Ke-Shu ZHOU
2
Author Information
1. Department of Hematology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, Henan Province, China.
2. Department of Hematology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, Henan Province, China E-mail: drzhouks77@163.com.
- Publication Type:Journal Article
- MeSH:
ATPases Associated with Diverse Cellular Activities/metabolism*;
Apoptosis;
Cell Cycle Proteins;
Cell Proliferation;
Humans;
Lymphoma, B-Cell;
Proto-Oncogene Proteins
- From:
Journal of Experimental Hematology
2021;29(5):1485-1492
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the regulatory effect of TRIP13 on the proliferation and apoptosis of B-cell lymphoma cells and its possible molecular mechanism by knocking down/overexpressing TRIP13 on the cell lines Granta-519 and JVM-2.
METHODS:Lentiviral transfection technology was used to construct Granta-519 and JVM-2 cells with knocked down or overexpressed TRIP13 and their control cells. The efficiency of transfection was determined by fluorescence microscopy. The efficiency of knockdown and overexpression was evaluated by real-time quantitative PCR and Western blot. The proliferation was detected by CCK-8 assay. The apoptosis was detected by the Annexin V-APC single staining. The cell cycle was detected by the PI staining. The expression levels of P53, MDM4, and BCL-2 were evaluated by Western blot.
RESULTS:After TRIP13 was knocked down, the proliferation ability of Granta-519 and JVM-2 cells was significantly reduced, and the apoptosis rate significantly increased. After TRIP13 was overexpressed, the proliferation ability of Granta-519 and JVM-2 cells was significantly enhanced, and the apoptosis was significantly reduced. After TRIP13 was knocked down, Granta-519 cells had obvious G
CONCLUSION:TRIP13 promotes the proliferation of B-cell lymphoma cells, inhibits their apoptosis, and affects their proliferation and apoptosis by participating in the regulation of the cell cycle. TRIP13 promotes the expression of BCL-2 proteins and inhibits the expression of MDM4 protein in B-cell lymphoma cells.
