The Establishment and Identification of Acute Myeloid Leukemia NOD-SCID-IL2rg
10.19746/j.cnki.issn.1009-2137.2021.05.010
- Author:
Wei-Ya ZHANG
1
;
Gao-Chun ZENG
1
;
Xiao-Mei CHEN
2
;
Su-Xia GENG
2
;
Yu-Lian WANG
2
;
Qiong LUO
2
;
Liu-Ping LUO
2
;
Pei-Long LAI
2
;
Jian-Yu WENG
2
;
Xin DU
3
Author Information
1. School of Medicine, South China University of Technology, Guangzhou 510006, Guangdong Province, China，Department of Hematology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
2. Department of Hematology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
3. School of Medicine, South China University of Technology, Guangzhou 510006, Guangdong Province, China，Department of Hematology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China ,E-mail: miyadu@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Disease Models, Animal;
Interleukin Receptor Common gamma Subunit;
Leukemia, Myeloid, Acute;
Luciferases/genetics*;
Male;
Mice;
Mice, Inbred NOD;
Mice, SCID
- From:
Journal of Experimental Hematology
2021;29(5):1429-1435
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the in vivo traceable acute myeloid leukemia mice model with Luciferase-Expressing KG1a Cells.
METHODS:KG1a cells with stable luciferase gene expression (called as KG1a-Luc cells) were constructed by lentivirus transfection, then sifted out by puromycin. Eighteen male NOD-SCID-IL2rg
RESULTS:KG1a cells expressing luciferase stably were successfully obtained. The tumor luminescence wildly spread at day 17 captured by in vivo imaging. The KG1a-Luc tumor cells could be detected in the peripheral blood of the mice, with the average percentage of (16.27±6.66)%. The morphology and pathology result showed that KG1a-Luc cells infiltrate was detected in bone marrow, spleens and livers. The survival time of the KG1a-Luc mice was notably shorter as compared with those in the control group, the median survival time was 30.5 days (95%CI: 0.008-0.260).
CONCLUSION:The acute myeloid leukemia NOD-SCID-IL2rg
