The Relationship between HIF1α and WTAP Expression Level in t(8;21) Acute Myeloid Leukemia.
10.19746/j.cnki.issn.1009-2137.2021.05.009
- Author:
Yang-Liu SHAO
1
,
2
;
Ze CHEN
3
;
Li-Li WANG
4
;
Dai-Hong LIU
4
;
Xiao-Ning GAO
5
Author Information
1. Department of Hematology, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China Medical School of Chinese PLA，Beijing 100853，China
2. Chinese PLA Unit 31647，Guigang 537100，Guangxi Zhuang Autonomous Region, China.
3. Department of Hematology, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China Medical School of Chinese PLA，Beijing 100853，China.
4. Department of Hematology, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.
5. Department of Hematology, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China E-mail: gaoxn@263.net.
- Publication Type:Journal Article
- MeSH:
Cell Cycle Proteins;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
Leukemia, Myeloid, Acute/genetics*;
Nuclear Proteins;
RNA Splicing Factors;
RNA, Messenger
- From:
Journal of Experimental Hematology
2021;29(5):1424-1428
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between hypoxia inducible factor 1 (HIF1α) and Wilms' tumor 1associating protein (WTAP) expression level in t(8;21) acute myeloid leukemia cells.
METHODS:The t(8;21) acute myeloid leukemia cell lines, including SKNO-1 and Kasumi-1 were treated by Echinomycin for 24 h, RT-qPCR and Western blot were used to detect the expression levels of WTAP mRNA and the protein. The CoCl
RESULTS:The expression level of WTAP mRNA and the protein in the echinomycin treated group was significantly lower than those in the control group (P<0.01). The expression level of WTAP protein in the CoCl
CONCLUSION:The inhibition of HIF1-α could down-regulates the expression of WTAP, while the up-regulation of HIF1α could up-regulates the expression of WTAP, which shows that there is a positive correlation of HIF1α and WTAP expression. This result suggesting that HIF1α may be involves in the expression regulation of WTAP gene.
