The Effect of Novel Phosphodiesterase 4 Inhibitor ZL-n-91 to the Proliferation of Leukemia Cells.
- Author:
Ping MAO
1
;
Zheng-Gang ZHAO
1
;
Lan WANG
1
;
Yu-Yu LI
1
;
Mei-Rong LI
1
;
Su-Jin ZHOU
1
;
Xin-Dan ZHANG
1
;
Yu WANG
1
;
Fang-Hong LI
1
;
Zi-Jian ZHAO
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; Humans; K562 Cells; Leukemia; Mice; Mice, Nude; Phosphodiesterase 4 Inhibitors/pharmacology*
- From: Journal of Experimental Hematology 2021;29(5):1387-1393
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibitory effects of novel phosphodiesterase 4 inhibitor ZL-n-91 to the proliferation of leukemia cells L1210 and K562.
METHODS:CCK-8 method was used to detect the effect of ZL-n-91 to the proliferation of L1210 and K562 cells, and the proliferation rate, IC
RESULTS:ZL-n-91 showed a significant inhibitory effect to the proliferation of leukemia cells L1210 and K562 in a dose-dependent manner (P<0.001). After treated by ZL-n-91, the leukemia cells L1210 and K562 in the S-phase in cell cycle decreased significantly compared with those in control group (P<0.01). The apoptosis of leukemia cells L1210 and K562 could be induced by ZL-n-91 (P<0.001), and the expression level of apoptosis related protein BAX significantly increased. In the animal experiment, the result showed that ZL-n-91 could significantly inhibit the growth of subcutaneously transplantation tumor (P<0.05).
CONCLUSION:The novel phosphodiesterase 4 inhibitor ZL-n-91 can effectively inhibit the proliferation of leukemia cells L1210 and K562, which has the potential of anti-leukemia drug development.
