Establishment of AML Mouse Model by Transplantation of Hematopoietic Cells from MLL-AF9 Transgenic Mice.
10.19746/j.cnki.issn.1009-2137.2021.05.001
- Author:
Wen-Yue LONG
1
,
2
;
Xing SHEN
3
;
Shuang XING
3
;
Guo-Lin XIONG
3
;
Hui-Guo WANG
4
;
Zu-Yin YU
5
Author Information
1. Life Science and Technology College of Dalian University, Dalian 116622, Liaoning Province, China
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.
3. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.
4. Life Science and Technology College of Dalian University, Dalian 116622, Liaoning Province, China E-mail: wanghuiguo163@163.com.
5. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China E-mail: yuzy79@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Hematopoietic Stem Cell Transplantation;
Leukemia, Myeloid, Acute;
Mice;
Mice, Inbred C57BL;
Mice, Transgenic;
Oncogene Proteins, Fusion
- From:
Journal of Experimental Hematology
2021;29(5):1369-1374
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a leukemia mouse model induced by transplantation of hematopoietic cells from mixed lineage leukemia (MLL)-AF9 transgenic mice so as to provide the basis for the mechanism research and drug screening of acute myeloid leukemia (AML).
METHODS:MLL-AF9 knock-in mice were bred and identified. When the mice developed leukemia, white blood cell (WBC) count in peripheral blood, flow cytometry and morphology method were analyzed to identify the disease. When the WBC count in peripheral blood was more than 100×10
RESULTS:The natural onset times of leukemia on MLL-AF9 knock-in mice were 22-28 weeks. The spleens of the transgenic mice enlarged and the bone marrow showed the immature forms of myeloid leukemia cells. Both the bone marrow and spleen cells highly expressed myeloid markers, CD11b and Gr-1. At least 0.5×10
CONCLUSION:The leukemia model of hematopoietic cell transplantation based on MLL-AF9 transgenic mice is successfully established, which can be used for the study of the pathogenesis and evaluation of therapeutic effect of AML.
