Protective effect of α-asarone and β-asarone on Aβ -induced inflammatory response in PC12 cells and its.
10.3724/zdxbyxb-2021-0162
- Author:
Jianhong SHI
1
;
Ruizhi LI
1
;
Yuanxiao YANG
1
;
Liting JI
1
;
Changyu LI
1
Author Information
1. 3. School of Pharmacy, Hangzhou Medical College, Hangzhou 310053, China.
- Publication Type:Journal Article
- Keywords:
Alzheimer’s disease;
Inflammation;
JNK/MAPK pathway;
Oxidation factor;
PC12 cell;
Vasoactive intestinal peptide;
α-Asarone;
β-Asarone
- MeSH:
Allylbenzene Derivatives;
Animals;
Anisoles/pharmacology*;
Apoptosis;
PC12 Cells;
Rats
- From:
Journal of Zhejiang University. Medical sciences
2021;50(5):591-600
- CountryChina
- Language:English
-
Abstract:
To investigate effects of α-asarone and β-asarone on induced PC12 cell injury and related mechanisms. Aβ toxic injury cell model was induced by Aβ in PC12 cells. PC12 cells were divided into blank control group, model control group, α-asarone group (0.5, 1.0, β-asarone group (6.3, 12.5, vasoactive intestinal peptide (VIP) group, and VIP antagonist control group. Cell survival rate was detected by CCK-8 kit; cell apoptosis rate was detected by flow cytometry. The levels of inflammatory cytokines interleukin (IL)-1, , tumor necrosis factor (TNF)-α, oxidation-related inducible nitric oxide synthase (iNOS), nitric oxide (NO), apoptosis factors caspase-3 and p53 were detected by ELISA method. The expressions of C-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK) were detected by Western blotting. Compared with model control group, cell survival rates of group, β-asarone group and VIP group increased; the cell apoptosis rate decreased; levels of apoptosis-related factors caspase-3, p53, inflammatory factors IL-1, TNF-α decreased; IL-10 level increased; levels of oxidization-related factors iNOS and NO decreased; the expression of JNK and p38MAPK protein decreased (all <0.05). After VIP antagonist intervention, the survival rate of β-asarone group decreased; apoptosis rate increased; apoptosis related factors caspase-3, p53, inflammatory factors IL-1, TNF-α increased; IL-10 decreased; oxidation related factors iNOS and NO increased; the expression of JNK and p38MAPK protein increased (all <0.05); while there were no significant changes in these indicators of α-asarone group (all >0.05). α-asarone and β-asarone have protective effects on PC12 cell injury induced by Aβ. β-asarone may inhibit inflammatory factors and oxidation-related factors through promoting VIP secretion, regulating JNK/MAPK pathway, and reducing PC12 cell apoptosis; however, the effect of α-asarone may be not related to VIP secretion.
