Role of the LRP1-pPyk2-MMP9 pathway in hyperoxia-induced lung injury in neonatal rats.
10.7499/j.issn.1008-8830.2108125
- Author:
Ya-Fei ZHENG
1
;
Hai-Yan ZHU
1
;
Wei WANG
1
;
Jing-Jing HU
1
;
Tian-Ping BAO
1
;
Zhao-Fang TIAN
1
Author Information
1. Department of Neonatology, First Huai'an Hospital Affiliated to Nanjing Medical University/Pediatric Diagnosis and Treatment Respiratory Key Laboratory of Huai'an, Huai'an, Jiangsu 223300, China.
- Publication Type:Journal Article
- Keywords:
Hyperoxia;
Low-density lipoprotein receptor-related protein 1;
Lung injury;
Matrix metalloproteinase;
Rat
- MeSH:
Animals;
Animals, Newborn;
Hyperoxia/complications*;
Lung;
Lung Injury/etiology*;
Matrix Metalloproteinase 9/genetics*;
Rats
- From:
Chinese Journal of Contemporary Pediatrics
2021;23(12):1289-1294
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To study the role of the low-density lipoprotein receptor-related protein 1 (LRP1)-proline-rich tyrosine kinase 2 phosphorylation (pPyk2)-matrix metalloproteinases 9 (MMP9) pathway in hyperoxia-induced lung injury in neonatal rats.
METHODS:A total of 16 neonatal rats were randomly placed in chambers containing room air (air group) or 95% medical oxygen (hyperoxia group) immediately after birth, with 8 rats in each group. All of the rats were sacrificed on day 8 of life. Hematoxylin and eosin staining was used to observe the pathological changes of lung tissue. ELISA was used to measure the levels of soluble LRP1 (sLRP1) and MMP9 in serum and bronchoalveolar lavage fluid (BALF). Western blot was used to measure the protein expression levels of LRP1, MMP9, Pyk2, and pPyk2 in lung tissue. RT-PCR was used to measure the mRNA expression levels of LRP1 and MMP9 in lung tissue.
RESULTS:The hyperoxia group had significantly higher levels of sLRP1 and MMP9 in serum and BALF than the air group (
CONCLUSIONS:The activation of the LRP1-pPyk2-MMP9 pathway is enhanced in hyperoxia-induced lung injury in neonatal rats, which may be involved in the pathogenesis of bronchopulmonary dysplasia.
