Protective effect of adrenomedullin on hyperoxia-induced lung injury.
10.7499/j.issn.1008-8830.2109005
- Author:
Min ZHANG
1
;
Li-Hua CHENG
1
;
Xiao-Tong YIN
1
;
Hao LUO
1
;
Cheng CAI
1
Author Information
1. Department of Neonatology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200062, China.
- Publication Type:Journal Article
- Keywords:
Adrenomedullin;
Extracellular signal-regulated kinase;
Human pulmonary microvascular endothelial cell;
Hyperoxia-induced lung injury;
Protein kinase B
- MeSH:
Adrenomedullin/genetics*;
Endothelial Cells;
Humans;
Hyperoxia/complications*;
Lung Injury;
Receptor Activity-Modifying Proteins
- From:
Chinese Journal of Contemporary Pediatrics
2021;23(12):1282-1288
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To study the role of adrenomedullin (ADM) in hyperoxia-induced lung injury by examining the effect of ADM on the expression of calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2), extracellular signal-regulated kinase (ERK), and protein kinase B (PKB) in human pulmonary microvascular endothelial cells (HPMECs) under different experimental conditions.
METHODS:HPMECs were randomly divided into an air group and a hyperoxia group (
RESULTS:Compared with the air group, the hyperoxia group had significant increases in the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB (
CONCLUSIONS:ERK1/2 and PKB may be the downstream targets of the ADM signaling pathway. ADM mediates the ERK/PKB signaling pathway by regulating CRLR/RAMP2 and participates in the protection of hyperoxia-induced lung injury.
