Hb Bart's Quantitative Analysis in the Screening of α-Thalassemia.
10.19746/j.cnki.issn.1009-2137.2021.06.036
- Author:
Song YI
1
;
Bo WANG
1
;
Hui LI
1
;
Hao ZHANG
2
;
Jie-Ping SONG
3
Author Information
1. Department of Clinical Laboratory Examination, Hubei Maternal and Child Health Hospital, Wuhan 430070, Hubei Province, China.
2. Zhejiang Biosan Biochemical Technologies Co.,Ltd, Hangzhou 310012, Zhejiang Province, China.
3. Department of Clinical Laboratory Examination, Hubei Maternal and Child Health Hospital, Wuhan 430070, Hubei Province, China,E-mail:songjieping@hbfy.com.
- Publication Type:Journal Article
- MeSH:
Hemoglobins, Abnormal/genetics*;
Heterozygote;
Humans;
Infant, Newborn;
Polymerase Chain Reaction;
alpha-Thalassemia/genetics*
- From:
Journal of Experimental Hematology
2021;29(6):1903-1906
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To research the relationship between difference types of α-thalassemia gene types and Hb Bart's hemoglobin bands.
METHODS:Capillary electrophoresis was used to screen thalassemia gene for the newborn form January 2020 to December 2020, and the thalassemia gene was detected by PCR or PCR-NGS in the positive patients. The relationship between α-thalassemia gene and Hb Bart's hemoglobin was compared and analyzed statistically.
RESULTS:There were significant differences in Hb Bart's hemoglobin among the different α-thalassemia mutation types, Hb Bart's was the highest in --SEA/-α
CONCLUSION:The Hb Bart's content of different genotypes of α-thalassemia are significantly different. The Hb Bart's content shows high application value in α-thalassemia screening and genotyping identification.
