Elemene Emulsion Injection Administration Reduces Neuropathic Pain by Inhibiting Astrocytic NDRG2 Expression within Spinal Dorsal Horn.
10.1007/s11655-021-3438-3
- Author:
Li-Tian MA
1
;
Yang BAI
2
;
Jie LI
3
;
Yu QIAO
4
;
Yang LIU
5
;
Jin ZHENG
6
Author Information
1. Department of Traditional Chinese Medicine, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, China.
2. Department of Anatomy, Histology and Embryology & K. K. Leung Brain Research Centre, Fourth Military Medical University, Xi'an, 710032, China.
3. Department of Endocrinology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710038, China.
4. Laser Medical Center, Hainan Hospital, People's Liberation Army General Hospital, Sanya, Hainan Province, 572013, China.
5. Health Center of 31658 of the People's Liberation Army, Xining, 810000, China.
6. Department of Traditional Chinese Medicine, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, China. zjddln@163.com.
- Publication Type:Journal Article
- Keywords:
NMYC downstream-regulated gene 2;
astrocyte;
elemene emulsion injection
- MeSH:
Animals;
Astrocytes;
Disease Models, Animal;
Emulsions;
Hyperalgesia/drug therapy*;
Nerve Tissue Proteins;
Neuralgia/drug therapy*;
Rats;
Rats, Sprague-Dawley;
Sesquiterpenes;
Spinal Cord;
Spinal Cord Dorsal Horn
- From:
Chinese journal of integrative medicine
2021;27(12):912-918
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the mechanisms underlying elemene-induced analgesia in rats with spared nerve injury (SNI).
METHODS:Sixty-five rats were equally divided into 5 groups using a random number table: naive group, sham group, SNI group, SNI + elemene (40 mg·kg
RESULTS:The SNI rat model exhibited a significant decrease in paw withdrawal threshold and exploratory behaviour in the EPM (P<0.05). Consecutive administration of elemene alleviated SNI-induced mechanical allodynia and anxiety in rats (P<0.05). Immunohistochemical data showed that elemene decreased SNI-induced upregulation of NDRG2 within the SDH (P<0.05). Double immunofluorescent staining data further showed that elemene decreased SNI-induced upregulation of the number of GFAP immunoreactive (-ir), NDRG-ir, and GFAP/NDRG2 double-labelled cells within the SDH (P<0.05). Immunoblotting data showed that elemene decreased SNI-induced upregulation of GFAP and NDRG2 within the SDH (P<0.05).
CONCLUSION:Elemene possibly alleviated neuropathic pain by downregulating the expression of NDRG2 in spinal astrocytes in a rat model of SNI.
