A Single-cell Transcriptome Atlas of Cashmere Goat Hair Follicle Morphogenesis.
10.1016/j.gpb.2021.07.003
- Author:
Wei GE
1
;
Weidong ZHANG
1
;
Yuelang ZHANG
1
;
Yujie ZHENG
1
;
Fang LI
1
;
Shanhe WANG
2
,
3
;
Jinwang LIU
4
;
Shaojing TAN
5
;
Zihui YAN
5
;
Lu WANG
5
;
Wei SHEN
5
;
Lei QU
4
;
Xin WANG
6
Author Information
1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
2. Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
3. College of Animal Science & Technology, Yangzhou University, Yangzhou 225000, China.
4. Life Science Research Center, Yulin University, Yulin 719000, China.
5. College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
6. Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. Electronic address: xinwang5@nwsuaf.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cashmere goat;
Cellular heterogeneity;
Developmental trajectory;
Hair follicle morphogenesis;
Single-cell transcriptome
- From:
Genomics, Proteomics & Bioinformatics
2021;19(3):437-451
- CountryChina
- Language:English
-
Abstract:
Cashmere, also known as soft gold, is produced from the secondary hair follicles (SHFs) of cashmere goats. The number of SHFs determines the yield and quality of cashmere; therefore, it is of interest to investigate the transcriptional profiles present during cashmere goat hair follicle development. However, mechanisms underlying this development process remain largely unexplored, and studies regarding hair follicle development mostly use a murine research model. In this study, to provide a comprehensive understanding of cellular heterogeneity and cell fate decisions, single-cell RNA sequencing was performed on 19,705 single cells of the dorsal skin from cashmere goat fetuses at induction (embryonic day 60; E60), organogenesis (E90), and cytodifferentiation (E120) stages. For the first time, unsupervised clustering analysis identified 16 cell clusters, and their corresponding cell types were also characterized. Based on lineage inference, a detailed molecular landscape was revealed along the dermal and epidermal cell lineage developmental pathways. Notably, our current data also confirmed the heterogeneity of dermal papillae from different hair follicle types, which was further validated by immunofluorescence analysis. The current study identifies different biomarkers during cashmere goat hair follicle development and has implications for cashmere goat breeding in the future.
