Determination of β-lactoglobulin in Anti-HPV Biological Protein Dressing by Amino Acid Assay.
10.3969/j.issn.1671-7104.2021.05.017
- Author:
Xiaomeng ZHANG
1
;
Jun FU
1
;
Kangjun WANG
1
;
Jucai WANG
1
Author Information
1. Hainan Key Laboratory for Pharmaceutical Quality Research, Hainan Institute for Drug Control, Haikou, 570216.
- Publication Type:Journal Article
- Keywords:
amino acid assay;
anti-HPV biological protein dressing;
determination;
β-lactoglobulin
- MeSH:
Amino Acids;
Bandages;
Lactoglobulins
- From:
Chinese Journal of Medical Instrumentation
2021;45(5):551-554
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an amino acid assay for the determination of β-lactoglobulin in Anti-HPV biological protein dressing.
METHODS:Under acidic conditions, β-lactoglobulin is hydrolyzed into free amino acids, separated by cation exchange chromatography, and derivatived after ninhydrin column. The chromatogram at 570 nm is collected. The content of β-lactoglobulin in the sample is indirectly determined by measuring the lysine content obtained by hydrolysis.
RESULTS:β-lactoglobulin has a good linear relationship in the concentration range of 77.28~309.12 μg/mL (
CONCLUSIONS:The method is simple, specific, accurate and reproducible, which is suitable for the quantitative analysis of β-lactoglobulin in anti-HPV biological protein dressing.
