Application of mapping allele with resolved carrier status technique for preimplantation genetic testing in carriers with balanced chromosomal translocations.
10.3760/cma.j.cn511374-20200629-00479
- VernacularTitle:等位基因映射识别胚胎平衡易位携带状态在植入前遗传学检测中的应用
- Author:
Qiuxiang HUANG
1
,
2
;
Yun LIU
;
Chunli LIN
;
Zhibiao CHEN
;
Zhiren LIU
;
Wujian HUANG
;
Lihua MAO
;
Lingyun HE
;
Zhihong WANG
Author Information
1. Department of Obstetrics & Gynecology, Center of Reproductive Medicine, Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Team)/ Dongfang Hospital Affiliated to Xiamen University (900th Hospital of the Joint Logistics Team), Fuzhou, Fujian 350025, China. liuyunfj@126.com
2. xiaonvwang@163.com.
- Publication Type:Journal Article
- MeSH:
Alleles;
Aneuploidy;
Blastocyst;
Female;
Fertilization in Vitro;
Genetic Testing;
Humans;
Pregnancy;
Preimplantation Diagnosis;
Retrospective Studies;
Translocation, Genetic
- From:
Chinese Journal of Medical Genetics
2021;38(9):845-848
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To assess the application value of mapping allele with resolved carrier status (MaReCs) technique for preimplantation genetic testing (PGT).
METHODS:The characteristics of MaReCs for PGT and outcome of patients were retrospectively analyzed.
RESULTS:Compared with those who could not use the technique, carriers who have used the MaReCs technique were younger, had significantly higher level of anti-Mullerian hormone, more antral follicles, occytes, mature occytes, biopsied embryos and euploid embryos, and lower risks for de novo chromosomal abnormality (P<0.05). It was necessary for couples with fewer oocytes, mature oocytes and balstocyst to preserve discarded embryos to facilitate the test. Carriers who have used the MaReCs technique had higher clinical pregnancy rate and abortion rate compared with those undergoing routine PGT, albeit no significant difference was found between the two groups (P> 0.05). Carriers undergoing MaReCs test could preferentially select embryos with normal chromosome structures for the transfer.
CONCLUSION:Application of MaReCs has a prerequisite for having a minimum number of occytes and biopsied embryos and using discarded embryos sometimes. MaReCs is efficient for the detection of carrier status of embryos and attaining higher rate of pregnancy and live birth, which can significantly improve the outcome for couples carrying chromosomal translocations.
