Lentivirus mediated siRNA hsa-circ-0000885 transfection of BMSCs and osteoclast co-culture system on cell differentiation, proliferation and apoptosis.
10.12200/j.issn.1003-0034.2021.10.017
- Author:
You-Shun ZHAO
1
;
Ping LIN
1
;
Ying-Chun TU
1
;
Tao AN
1
;
Yu-Ping WU
1
;
Xiao-Fei LI
1
Author Information
1. Department of Bone Surgery, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, the Jinhua Municipal Central Hospital, Jinhua 321000, Zhejiang, China.
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Osteoclast;
Osteoporosis;
circRNA
- MeSH:
Aged;
Apoptosis/genetics*;
Cell Differentiation;
Cell Proliferation/genetics*;
Cells, Cultured;
Coculture Techniques;
Female;
Humans;
Lentivirus;
Leukocytes, Mononuclear;
Mesenchymal Stem Cells;
Middle Aged;
Osteoclasts;
Osteogenesis/genetics*;
RNA, Small Interfering/genetics*;
Transfection
- From:
China Journal of Orthopaedics and Traumatology
2021;34(10):978-984
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of siRNA hsa-circ-0000885 modified bone marrow mesenchymal stem cells (BMSCs) on osteogenic differentiation, cell proliferation and apoptosis in order to provide new ideas and methods for the clinical treatment of osteoporosis (OP).
METHODS:From September 2018 to February 2020, 13 patients with osteoporosis admitted to our hospital were selected as the research objects, including 11 females and 2 males, with an age of (65.45±10.77) years old. After obtaining the informed consent of patients, peripheral blood tissues were extracted. Then the expression level of cir-cRNA in peripheral blood mononuclear cells(PBMC) was detected by circ RNA chip. The expression of circ RNA was silenced by siRNA technology. The BMSCs were transfected with lentivirus. According to the siRNA interference plasmid hsa-circ-0000885, the cells were divided into the blank group, the empty vector group and the siRNA interference group. After 72 hours of treatment, the cell cycle was detected by flow cytometry, the apoptosis level was detected by AV-PI kit, and the osteogenic differentiation ability of BMSCs was detected by ALP staining.
RESULTS:The expression of hsa-circ-0000885 in PBMC of patients with osteoporosis was significantly higher than that of healthy controls (
CONCLUSION:The lentivirus mediated siRNA hsa-circ-0000885 plasmid transfected into BMSCs and osteoclast co culture system can promote cell proliferation, inhibit apoptosis and promote osteogenic differentiation of BMSCs, which can be used as a potential therapeutic target for OP patients.
