Tobramycin promotes fracture healing by upregulating expressions of ALP and RUNX2 proteins through activating Wnt/β-catenin pathway.
10.12200/j.issn.1003-0034.2021.09.015
- Author:
Hong-Gang CUI
1
Author Information
1. The Third Department of Orthopaedics, the 7th People's Hospital of Zhengzhou, Zhengzhou 450000, Henan, China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
Fracture healing;
Osteogenesis;
Tobramycin;
Wnt/β-catenin signaling pathway
- MeSH:
Alkaline Phosphatase;
Animals;
Cell Differentiation;
Core Binding Factor Alpha 1 Subunit/genetics*;
Femoral Fractures;
Fracture Healing;
Male;
Osteogenesis;
Rats;
Tobramycin;
Wnt Signaling Pathway;
beta Catenin/metabolism*
- From:
China Journal of Orthopaedics and Traumatology
2021;34(9):866-869
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore effect of tobramycin (TOB) on healing of femoral fractures in rats.
METHODS:Totally 32 male sprague-dawley (SD) rats were selected and randomly divided into sham group (group A), fracture group (group B), fracture with TOB group (group C) and fracture + TOB + IWR-1 group (group D), 8 rats in each group. Close femoral fracture model in rats were established in group B, C and D, group A was sham operation without otherwise process. Group D was intraperitoneal injected 100 μl (8 μM) of Wnt pathway inhibitor IWR-1-endo (IWR-1) before molding at 1 day. At 1 day after molding, 100 μl (100 μM) of TOB was intraperitoneally injected into group C and D at once a day for 7 days. At 7 weeks after modling, fracture healing of group B, C and D were observed by X-ray, Western blotting was appilied to detect alkaline phosphatase(ALP) and Runt related transcription factor 2 (RUNX2) and β-catenin of Wnt passway.
RESULTS:X-ray results showed fracture line disappeared, callus formation and fracture healing well in group C compared with begning of molding; while a little fracture line, callus formation and fracture malunion in group B and d could be seen. Western blotting results showed ALP, RUNX2 and expression of β-catenin in group B, C and D were higher than that of group A (
CONCLUSION:Tobramycin could promote osteoblast differentiation and fracture healing by stimulating Wnt / β-catenin signaling pathway, up regulating expression of ALP and RUNX2.
