Altered Colonic Transit in TNBS-induced Experimental Colitis in Guinea Pig and Distribution of Nitric Oxide Synthase in the Colonic Wall.
- Author:
Seung Hyun CHO
1
;
Hyo Jin PARK
;
Jun Pyo CHUNG
;
Young Ho LEE
;
Sang Won JI
;
Tae Woong NO
;
Sang In LEE
Author Information
1. Departments of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. HJPARK21@yumc.yonsei.ac.kr
- Publication Type:Original Article ; English Abstract
- Keywords:
Colonic transit;
Colitis;
Nitric oxide synthase;
Trinitrobenzene sulfonic acid
- MeSH:
Animals;
Colitis/chemically induced/enzymology/*physiopathology;
Colon/*enzymology/innervation;
English Abstract;
*Gastrointestinal Transit;
Guinea Pigs;
Male;
Myenteric Plexus/*enzymology;
Nitric-Oxide Synthase/*metabolism;
Trinitrobenzenesulfonic Acid
- From:The Korean Journal of Gastroenterology
2004;44(6):308-313
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND/AIMS: Inflammation-induced alterations in smooth muscle contractility may be due to the effects on smooth muscle itself, neurotransmitters or enteric nerves. In dextran sulfate sodium-induced colitic rat, the delay in colonic transit was caused by decreased activity and production of neuronal nitric oxide synthase (nNOS) in the myenteric plexus of the distal colon. The aim of this study was to investigate the relationship between the delay in colonic transit and the distribution of inducible NOS (iNOS) and nNOS immunoreactive cells in the myenteric plexus of trinitrobenzene sulfonic acid (TNBS)-induced colitic guinea pig. METHODS: Sacrificed and their colonic tissues of forty-five TNBS-induced colitic guinea pigs were used to measure the colonic transit, and analyzed by immunohistochemistry. RESULTS: Colonic transit was delayed significantly at 3, 7 and 14 days after administration of TNBS. In control, nNOS immunoreactivity was present in the mucosa, submucosa, lamina propria, and ganglion cells of the myenteric plexus, while after TNBS treatment, reduced nNOS cells were found. However, the number of nNOS ganglion cells in the myenteric plexus was similar to those seen in controls. After administration of TNBS, iNOS immunoreactivity was increased in the mucosa and submucosa, but the number of iNOS positive ganglion cells in the myenteric plexus was not changed compared to control. CONCLUSIONS: It is suggested that in TNBS-induced guinea pig colitis, delayed colonic transit is not associated with the expression of nNOS nor iNOS in the myenteric plexus.
