Anti-interference hemoglobin analysis system by high performance liquid chromatography.
10.7507/1001-5515.202101069
- Author:
Yan XU
1
;
Tiantian YAO
2
;
Wenyong HU
2
;
Bo ZHANG
2
;
Xingming GUO
1
Author Information
1. College of Bioengineering, Chongqing University, Chongqing 400044, P.R.China.
2. Shenzhen Lifotronic of Technology Co., Ltd., Shenzhen, Guangdong 518055, P.R.China.
- Publication Type:Journal Article
- Keywords:
glycated hemoglobin;
hemoglobin variant;
high performance liquid chromatography;
thalassemia factor HbF/HbA2
- MeSH:
Chromatography, High Pressure Liquid;
Fetal Hemoglobin/analysis*;
Glycated Hemoglobin A/analysis*;
Hemoglobin A2/analysis*;
Hemoglobins
- From:
Journal of Biomedical Engineering
2021;38(5):940-950
- CountryChina
- Language:Chinese
-
Abstract:
High performance liquid chromatography (HPLC) is currently the mainstream technology for detecting hemoglobin. Glycated hemoglobin (HbA1c) is a gold indicator for diagnosing diabetes, however, the accuracy of HbA1c test is affected by thalassemia factor hemoglobin F (HbF)/hemoglobin A2 (HbA2) and variant hemoglobin during HPLC analysis. In this study, a new anti-interference hemoglobin analysis system of HPLC is proposed. In this system, the high-pressure three-gradient elution method was improved, and the particle size and sieve plate aperture in the high-pressure chromatography column and the structure of the double-plunger reciprocating series high-pressure pump were optimized. The system could diagnose both HbA1c and thalassemia factor HbF/HbA2 and variant hemoglobin, and the performance of the system was anti-interference and stable. It is expected to achieve industrialization. In this study, the HbA1c and thalassemia factor HbF/HbA2 detection performance was compared between this system and the world's first-line brand products such as Tosoh G8, Bio-Rad Ⅶ and D10 glycosylated hemoglobin analysis system. The results showed that the linear correlation between this system and the world-class system was good. The system is the first domestic hemoglobin analysis system by HPLC for screening of HbA1c and thalassemia factor HbF/HbA2 rapidly and accurately.
