Pharmacokinetics of eight active ingredients of Salviae Miltiorrhizae Radix et Rhizoma-Puerariae Lobatae Radix combination in rats.
10.19540/j.cnki.cjcmm.20210506.201
- Author:
Xiao-Yan LIU
1
;
Xiao-Yuan LI
2
;
Xu CHEN
3
;
Zhi-Fang HUANG
2
;
Yun-Hua LIU
2
;
Yu-Hong LIU
2
;
Yan CHEN
2
;
Zheng-Huai TAN
2
;
Jin-Hai YI
4
Author Information
1. Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China Southwest Medical University Luzhou 646000, China.
2. Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China.
3. Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
4. Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China Southwest Medical University Luzhou 646000, China Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
- Publication Type:Journal Article
- Keywords:
Dan′ge Fentong Extract;
Puerariae Lobatae Radix;
Salviae Miltiorrhizae Radix et Rhizoma;
pharmacokinetics;
rat
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/pharmacokinetics*;
Plant Roots/chemistry*;
Pueraria/chemistry*;
Rats;
Rhizome/chemistry*;
Salvia miltiorrhiza/chemistry*;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(24):6530-6541
- CountryChina
- Language:Chinese
-
Abstract:
To reveal the rationality of compatibility of Salviae Miltiorrhizae Radix et Rhizoma(SMRR) and Puerariae Lobatae Radix(PLR) from the perspective of pharmacokinetics, this study established a UPLC-MS/MS method for quantitative determination of PLR flavonoids(3'-hydroxy puerarin, puerarin, puerarin 6″-O-xyloside, 3'-methoxy puerarin, puerarin apioside) and salvianolic acids and tanshinones(salvianolic acid B, cryptotanshinone, and tanshinone Ⅱ_A) in plasma of rats. Rats were given SMRR extract, PLR extract, and SMRR-PLR extract by gavage and then plasma was collected at different time. UPLC separation was performed under the following conditions: Eclipse C_(18) column(2.1 mm×50 mm, 1.8 μm), 0.1% formic acid in water(A)-0.1% formic acid in acetonitrile(B) as mobile phase for gradient elution. Conditions for MS are as below: multiple reaction monitoring(MRM), ESI~(+/-). Comprehensive validation of the UPLC-MS/MS method(specifically, from the aspects of calibration curve, precision, accuracy, repeatability, stability, matrix effect, extract recovery) was performed and the result demonstrated that it complied with quantitative analysis requirements for biological samples. Compared with SMRR extract alone or PLR extract alone, SMRR-PLR extract significantly increased the AUC and C_(max) of PLR flavonoids and tanshinones in rat plasma, suggesting that the combination of SMRR and PLR promoted the absorption of the above components. The underlying mechanism needs to be further studied.
